Molecular and phenotypic characterization of the vancomycin-resistant gene in bacterial isolates acquired from catheter tips.

Molecular and phenotypic characterization of the vancomycin-resistant gene in bacterial isolates acquired from catheter tips. Cell Mol Biol (Noisy-le-grand). 2017 Dec 30;63(12):63-67 Authors: Siddiqui S, Saeed M, Faisal SM, Akhtar A, Sarim KM, Khan M, Ahmad I, Ahmad I Abstract Gram-positive bacteria, particularly Staphylococcus aureus is a significant pathogen, not only in the hospital setting but the community also. S. aureus is a major cause of serious hospital and community-acquired infections, particularly in the colonized individuals. The emergence of vancomycin-resistant S. aureus (VRSA) strains has led to global concerns about treatments for staphylococcal infections. Until now, few strains of VRSA have been reported worldwide. The conventional disk diffusion method for determination of vancomycin sensitivity often misclassifies intermediately susceptible isolates to fully sensitive. However, non-automated minimum inhibitory concentration (MIC) detection methods are the gold standards. Hence there is a dire need of some advanced methods for rapid detection of VRSA strains. In the present study, Gram-positive clinical isolates were collected from different wards of K.G.M.U.  Hospital, among them, 12 bacterial isolates were identified as Staphylococcus aureus and 18 isolates as Klebsiella spp. Genomic DNA of S. aureus was isolated and used as template in PCR for detection of the presence of van A and van X gene based on a given protocol. Nosocom...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

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Authors: Bae E, Kim CK, Jang JH, Sung H, Choi Y, Kim MN Abstract BACKGROUND: No study has examined the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) bacteremia in Korean veterans' hospitals. We investigated the microbiological and clinical epidemiology of S. aureus bacteremia at the central Veterans Health Services (VHS) hospital in Korea. METHODS: Patients with S. aureus bacteremia were consecutively enrolled from February to August 2015. Bacteremia was classified as hospital-acquired (HA), community-onset healthcare-associated (COHA), or community-acquired (CA). MRSA bacteremia risk facto...
Source: Annals of Laboratory Medicine - Category: Laboratory Medicine Tags: Ann Lab Med Source Type: research
Publication date: Available online 16 November 2018Source: Journal of Hospital InfectionAuthor(s): Vicente Pintado, Rosario Pazos, Manuel Enrique Jiménez-Mejías, Azucena Rodríguez-Guardado, Beatriz Díaz-Pollán, Carmen Cabellos, Juan Manuel García-Lechuz, Jaime Lora-Tamayo, Pere Domingo, Elena Muñez, Diego Domingo, Fernando González-Romo, José Antonio Lepe-Jiménez, Carlos Rodríguez-Lucas, Antonio Gil, Iván Pelegrín, Fernando Chaves, Virginia Pomar, Antonio Ramos, Teresa AlarcónSummaryBackgroundStaphylococcus aureus meningitis i...
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
ConclusionThis study shows low resistance to meropenem and tigecycline among the Enterobacteriaceae, and continued activity of linezolid, vancomycin and tigecycline against Gram-positive organisms. However, antimicrobial resistance continues to be problematic in Eastern Europe and requires continued surveillance.
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Methicillin resistant Staphylococcus aureus (MRSA) is recognized worldwide as a leading cause of hospital and community infections. Biofilm formation by MRSA is an extremely important virulence factor to be under...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
ConclusionsWhole genome sequencing allowed us to study the epidemiology and genomic repertoire ofS. aureus in a clinical setting and provided evidence of its often underestimated complexity. Some virulence factors and clones are specific of disease types, but the variability and dispensability of many antigens considered for vaccine development together with the quickly changing epidemiology ofS. aureus makes it very challenging to develop full-coverage therapies and vaccines. Expanding WGS-based surveillance ofS. aureus to many more hospitals would allow the identification of specific strains representing the main burden ...
Source: Genome Medicine - Category: Genetics & Stem Cells Source Type: research
van A Abstract Melaleuca alternifolia tea tree oil (TTO) is largely used in cutaneous infections. Clinical observations reported antibacterial, antifungal, and antiviral activities, whereas in vitro experiments ascribed most of biological properties to terpinen-4-ol. Since different plant chemotypes and storage conditions result in variations of chemical composition of commercially available TTO, in this study we investigated the antimicrobial activity and the chemical profile of ten commercially available TTO products. The antimicrobial activity was assessed against Candida glabrata, Herpes simplex virus type 1 (...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
Publication date: 10 January 2019Source: International Journal of Pharmaceutics, Volume 554Author(s): Satoshi Uchiyama, Samira Dahesh, Victor Nizet, Jack KesslerAbstractStaphylococcus aureus, a leading cause of serious human infections in both healthcare and community settings, is increasingly difficult to control due to expanding resistance to multiple antibiotic classes. Methicillin-resistant S. aureus (MRSA) strains have disseminated on a global scale and are associated with adverse patient outcomes, increased hospital stays, and significant economic costs to the healthcare system. A proximal step in S. aureus infection...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
Conclusion: TROAI are relatively uncommon in children but cause significant morbidity. While MRSA contributes substantially to TROAI, the highly diverse microbiology emphasizes the importance of obtaining cultures for microbiologic diagnosis and management. Clinicians should be aware of the frequency of E. cloacae in TROAI and its high risk for sequelae.
Source: The Pediatric Infectious Disease Journal - Category: Infectious Diseases Tags: Original Studies Source Type: research
Conclusion : The result suggests that a broad-spectrum antibacterial compound obtained from halophilic actinomycetes is effective against pathogenic bacteria. This compound may be a good alternative treatment against antibiotic-resistant pathogen S. aureus. PMID: 30397580 [PubMed]
Source: BioImpacts - Category: Research Tags: Bioimpacts Source Type: research
In conclusion, PgTeL is a lectin with multiple inhibitory effects on S. aureus clinical isolates. PMID: 30414418 [PubMed - as supplied by publisher]
Source: International Journal of Biological Macromolecules - Category: Biochemistry Authors: Tags: Int J Biol Macromol Source Type: research
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