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Molecular and phenotypic characterization of the vancomycin-resistant gene in bacterial isolates acquired from catheter tips.

Molecular and phenotypic characterization of the vancomycin-resistant gene in bacterial isolates acquired from catheter tips. Cell Mol Biol (Noisy-le-grand). 2017 Dec 30;63(12):63-67 Authors: Siddiqui S, Saeed M, Faisal SM, Akhtar A, Sarim KM, Khan M, Ahmad I, Ahmad I Abstract Gram-positive bacteria, particularly Staphylococcus aureus is a significant pathogen, not only in the hospital setting but the community also. S. aureus is a major cause of serious hospital and community-acquired infections, particularly in the colonized individuals. The emergence of vancomycin-resistant S. aureus (VRSA) strains has led to global concerns about treatments for staphylococcal infections. Until now, few strains of VRSA have been reported worldwide. The conventional disk diffusion method for determination of vancomycin sensitivity often misclassifies intermediately susceptible isolates to fully sensitive. However, non-automated minimum inhibitory concentration (MIC) detection methods are the gold standards. Hence there is a dire need of some advanced methods for rapid detection of VRSA strains. In the present study, Gram-positive clinical isolates were collected from different wards of K.G.M.U.  Hospital, among them, 12 bacterial isolates were identified as Staphylococcus aureus and 18 isolates as Klebsiella spp. Genomic DNA of S. aureus was isolated and used as template in PCR for detection of the presence of van A and van X gene based on a given protocol. Nosocom...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

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Abstract Recently, the prevalence of staphylococcal cassette chromosome mec (SCCmec) type IV isolates, which are the major community-acquired methicillin-resistant Staphylococcus aureus (MRSA), have increased in Japanese hospitals. The aim of this study was to elucidate the detailed molecular epidemiological features of the SCCmec type IV clones in Japanese hospitals. When 2589 MRSA isolated from four hospitals in Tokyo, Japan between 2010 and 2014 were analysed, the proportion of SCCmec type IV overtook that of type II, which was the major type of hospital-acquired MRSA in 2014. Multilocus sequence typing showed ...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
Bacterial infections are either caused by gram-positive bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) or gram-negative bacteria such as Pseudomonas aeruginosa (PA). In previous studies conducted by my own group it has showed that photodynamic therapy (PDT) using 5-Aminolevulinic acid (ALA) has bactericidal effects against MRSA and PA individually. However, an infected ulcer usually carries both gram-positive and gram-negative bacteria simultaneously. Therefore, in this study we investigated the antibacterial effect of PDT against MRSA and PA doubly infected sample.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Photobiology Source Type: research
In this study, we demonstrate the novel efficacy of a small molecule compound to enhance clearance of methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Acinetobacter baumannii strains by primary human dermal fibroblasts in vitro.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Innate Immunity, Microbiology, Inflammation Source Type: research
CONCLUSION: Current MRSA decolonisation regimens are well tolerated and effective for MSSA decolonization for the anterior nares and groin. The decolonization effect is preserved for at least 10 days following treatment. PMID: 29671723 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research
Non-hospital residential facilities are important reservoirs for MRSA transmission. However, conclusions and public health implications drawn from the many mathematical models depicting nosocomial MRSA transmi...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
CONCLUSION: It was found that there is a reasonable correlation between the binding free energy and the antibacterial activity. PMID: 29663895 [PubMed - as supplied by publisher]
Source: Medicinal Chemistry - Category: Chemistry Authors: Tags: Med Chem Source Type: research
PREVALENCE AND ANTIBIOTIC SUSCEPTIBLITY OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS, COLLECTED AT THAMMASAT UNIVERSITY HOSPITAL, THAILAND, AUGUST 2012 - JULY 2015. Southeast Asian J Trop Med Public Health. 2017 Mar;48(2):351-9 Authors: Phokhaphan P, Tingpej P, Apisarnthanarak A, Kondo S Abstract We analyzed data of Staphylococcus aureus isolated from patients attending Thammasat University Hospital, Thailand from August 2012 to July 2015. In total, 232/502 (46%) S. aureus isolates were methicillin-resistant S. aureus (MRSA). There was a declining trend of proportion of MRSA infection, but the preval...
Source: Southeast Asian Journal of Tropical Medicine and Public Health - Category: Tropical Medicine Tags: Southeast Asian J Trop Med Public Health Source Type: research
The objectives of this study were to develop and determine the benefits of an Antibiotic Options Index (AOI); an index that summarizes antibiotic susceptibility data for a pathogen by presenting it as the availability of antibiotic treatment options. The AOI was calculated using antibiogram data for the seven most commonly isolated pathogens from the National Antimicrobial Resistance Surveillance Center of Thailand between 1998 and 2014 and was classified as acceptable (AOI ≥ 0.8) or unacceptable (AOI
Source: Southeast Asian Journal of Tropical Medicine and Public Health - Category: Tropical Medicine Tags: Southeast Asian J Trop Med Public Health Source Type: research
The Centers for Disease Control and Prevention (CDC) estimates that approximately 80,641 severe methicillin-resistant Staphylococcus aureus (MRSA) infections occur every year, leading to 11,285 deaths [1], while the healthcare cost of a single MRSA infection may be as high as $34,657 [2]. In turn, these challenges are largely attributed to an increase in multi-drug resistance among S. aureus strains due, at least in part, to inappropriate antibiotic use [3]. The increase of multi-drug resistant MRSA strains [4-6] has led the CDC to classify MRSA as a “serious” public health threat [1], given that MRSA infection...
Source: International Journal of Antimicrobial Agents - Category: Drugs & Pharmacology Authors: Source Type: research
Conclusions Patient privacy curtains became progressively contaminated with bacteria, including MRSA. Between days 10 and 14 after being hung, curtains showed increased MRSA positivity. This may represent an opportune time to intervene, either by cleaning or replacing the curtains.
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
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