Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition

Publication date: 9 January 2018 Source:Stem Cell Reports, Volume 10, Issue 1 Author(s): Auston Z. Miller, Alexander Satchie, Alex P. Tannenbaum, Aman Nihal, James A. Thomson, David T. Vereide Arterial diseases continue to pose a major health concern but in vitro studies are limited because explanted cells can exhibit poor proliferative capacity and a loss of specificity. Here, we find that two transcription factors, MYCN and SOX17, induce and indefinitely expand in culture precursors of human arterial endothelial cells (expandable arterial endothelial precursors [eAEPs]). The eAEPs are derived from CD34+ cells found in umbilical cord blood or adult bone marrow. Independent eAEP lines differ in their proclivity to undergo an endothelial-to-mesenchymal transition (EndoMT), a hallmark event in a broad array of vascular diseases and disorders. Some cell lines spontaneously become mesenchymal over time in culture, an effect exacerbated by inhibition of the fibroblast growth factor receptor, while others do not readily convert. These distinctions were exploited to identify genes that correlate with resistance to an EndoMT and to elucidate transcriptional changes that underpin the transition. Teaser Vereide and colleagues identify a means to induce and maintain human arterial endothelial precursors (eAEPs) in culture by using two transcription factors, MYCN and SOX17. Although the eAEPs facilitate a range of human arterial studies, in this study the authors examine the pro...

Source: Stem Cell Reports - January 9, 2018 Category: Stem Cells Source Type: research