Gene expression profiling provides insights into the immune mechanism of Plutella xylostella midgut to microbial infection.

In this study, expressions of the immune-related genes in the midgut of P. xylostella orally infected with Staphylococcus aureus, Escherichia coli and Pichia pastoris were profiled by RNA-seq and qRT-PCR approaches. The results revealed that the Toll, IMD, JNK and JAK-STAT pathways and possibly the prophenoloxidase activation system in P. xylostella could be activated by oral infections, and moricins, gloverins and lysozyme2 might act as important effectors against microorganisms. Subsequent knock-down of IMD showed that this gene was involved in regulating the expression of down-stream genes in the IMD pathway. Our work indicates that the Toll, IMD, JNK and JAK-STAT pathways may synergistically modulate immune responses in the P. xylostella midgut, implying a complex and diverse immune system in the midgut of insects.
PMID: 29305978 [PubMed - as supplied by publisher]

Source: Gene - January 3, 2018 Category: Genetics & Stem Cells Authors: Lin J, Xia X, Yu XQ, Shen J, Li Y, Lin H, Tang S, Vasseur L, You M Tags: Gene Source Type: research