Design, construction and evaluation of multi-epitope antigens for diagnosis of Lyme disease.

In this study, the chimeric proteins (A/C-2, A/C-4 and A/C-7.1) consisting of B-cell epitopes of outer surface proteins OspA and OspC from Borrelia genospecies prevalent in Eastern Slovakia, were designed, over-expressed in E. coli, and used to detect specific anti-Borrelia antibodies in serologically characterized sera from patients with Lyme-like symptoms to evaluate their diagnostic potential. RESULTS: Results showed that chimeras vary in their immuno-reactivity when tested with human sera. Compared with the results obtained from a two-tier test, the application of recombinant multi-epitope chimeric proteins as diagnosis antigens, produced fair agreement in the case of A/C-2 (0.20
Source: Annals of Agricultural and Environmental Medicine : AAEM - Category: Environmental Health Authors: Tags: Ann Agric Environ Med Source Type: research

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Publication date: 2019Source: Methods in Microbiology, Volume 46Author(s): Kakarla Raghava Reddy, Pattubala Adinaraya Reddy, Chandragiri Venkata Reddy, Nagaraj P. Shetti, Bhatula Babu, Koutavarapu Ravindranadh, Muthukonda Venkatakrishnan Shankar, Madhava C. Reddy, Sarvesh Soni, Shivalingegowda NaveenAbstractSuperparamagnetic iron oxide nanoparticles (SPIONs) are the most extensively used functional nanomaterials as antibacterial agents, and for other biomedical applications due to their unique physical, chemical, magnetic and biocompatibility properties. However, due to their small size, particles tend to aggregate by dipo...
Source: Methods in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 21 May 2019Source: Journal of BiotechnologyAuthor(s): Tianzhen Xiong, Pu jia, Jing Jiang, Yajun Bai, Taiping Fan, Xiaohui Zheng, Yujie CaiAbstractD-danshensu (D-DSS), extracted from the plant Salvia miltiorrhiza (Danshen), is widely used to treat cardiovascular and cerebrovascular diseases. Here we engineered Escherichia coli strains to produce D-DSS from catechol, pyruvate and ammonia by one-pot biotransformation. Tyrosin-phenol lyase (TPL), L-amino acid deaminase (aadL), D-lactate dehydrogenase (ldhD) and glucose dehydrogenase (gdh) genes were overexpressed in Escherichia coli strain. F...
Source: Journal of Biotechnology - Category: Biotechnology Source Type: research
Publication date: Available online 22 May 2019Source: Journal of Molecular BiologyAuthor(s): Shannon M. Doyle, Joel R. Hoskins, Andrea N. Kravats, Audrey L. Heffner, Srilakshmi Garikapati, Sue WicknerAbstractMembers of the Hsp90 and Hsp70 families of molecular chaperones are important for the maintenance of protein homeostasis and cellular recovery following environmental stresses, such as heat and oxidative stress. Moreover, the two chaperones can collaborate in protein remodeling and activation. In higher eukaryotes, Hsp90 and Hsp70 form a functionally active complex with Hop (Hsp90-Hsp70 organizing protein) acting as a ...
Source: Journal of Molecular Biology - Category: Molecular Biology Source Type: research
Authors: Esfahanian M, Ghasemzadeh MA, Razavian SMH Abstract Today, metal-organic frameworks (MOFs) for the release of drugs have been studied extensively. In this research, we have investigated the novel magnetic framework including Fe3O4@PAA@ZIF-8 for the delivery of ciprofloxacin (CIP) antibiotic. Moreover, the antibacterial activity of Fe3O4@PAA@ZIF-8 and Fe3O4@PAA@ZIF-8@CIP screened against Staphylococcus aureus ( S. aureus) and Escherichia coli ( E. coli) bacteria. The structure of the nano carrier, as well as the encapsulation of the drug in the framework was confirmed by the Fourier-transform infrared spect...
Source: Artificial Cells, Nanomedicine and Biotechnology - Category: Biotechnology Tags: Artif Cells Nanomed Biotechnol Source Type: research
Publication date: Available online 21 May 2019Source: Protein Expression and PurificationAuthor(s): Rong-Qing ZhaoAbstractThe SnRK1 (SNF1 related protein kinase 1), a plant homologue of SNF1 (Sucrose non-fermenting 1)/AMPK (AMP-activated protein kinase), is an important metabolic sensor involving in catabolic and anabolic processes. SnRK1 is essential for plant metabolism regulation and response to environmental stresses. The plant SnRK1 consists of one catalytic (α1/α2 subunit) and two regulatory subunits (β1/β2/β3 and γ/βγ subunits), and functions as a heterotrimeric complex. H...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Publication date: Available online 21 May 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Zhi-neng Hong, Jun Jiang, Jiu-yu Li, Ren-kou Xu, Jing YanAbstractThe current knowledge of bacterial migration is mainly derived from work using bare or Fe-coated quartz sands as porous media. However, mineral coatings on quartz by phyllosilicates and Al-oxides prevail in natural soils, and their effect on bacterial transport remains unknown. Herein, we systematically explored the transport of two bacterial pathogens (Escherichia coli and Staphylococcus aureus) through saturated bare quartz and those coated by kaolinite (K...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Publication date: Available online 22 May 2019Source: Biochimica et Biophysica Acta (BBA) - Proteins and ProteomicsAuthor(s): Abyson Joseph, Valakunja Nagaraja, Ramanathan NateshAbstractGre factors reactivate stalled elongation complexes by enhancing the intrinsic transcript cleavage activity of RNA polymerase. Previous work by us has shown that unlike in Escherichia coli (E.coli), Mycobacterium tuberculosis Gre factor is essential for its survival. Apart from their role in transcription regulation Gre factors have been implicated in stress response. A recent study has shown the role of E.coli GreA as a cellular chaperone,...
Source: Biochimica et Biophysica Acta (BBA) Proteins and Proteomics - Category: Biochemistry Source Type: research
ConclusionsThe clinical course of acute NE was similar in adults and children with high frequency of AKI as well as thrombocytopenia, but with full recovery of all patients.
Source: Pediatric Nephrology - Category: Urology & Nephrology Source Type: research
ConclusionsEven though the pathological examination performed on one native liver demonstrated areas of thrombotic microangiopathy, we cannot completely rule out that eculizumab may have worsened the liver lesions. Before the efficacy of eculizumab in STEC-HUS is formally demonstrated, physicians should stay cautious in its use.
Source: Pediatric Nephrology - Category: Urology & Nephrology Source Type: research
Publication date: December 2019Source: Nonlinear Analysis: Real World Applications, Volume 50Author(s): Kevin E.M. Church, Xinzhi LiuAbstractA time-delayed SIR model with general nonlinear incidence rate, pulse vaccination and temporary immunity is developed. The basic reproduction number is derived and it is shown that the disease-free periodic solution generically undergoes a transcritical bifurcation to an endemic periodic solution as the vaccination coverage drops below a critical level. Using numerical continuation and a monodromy operator discretization scheme, we track the bifurcating endemic periodic solution as th...
Source: Nonlinear Analysis: Real World Applications - Category: Research Source Type: research
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