Design, construction and evaluation of multi-epitope antigens for diagnosis of Lyme disease.

In this study, the chimeric proteins (A/C-2, A/C-4 and A/C-7.1) consisting of B-cell epitopes of outer surface proteins OspA and OspC from Borrelia genospecies prevalent in Eastern Slovakia, were designed, over-expressed in E. coli, and used to detect specific anti-Borrelia antibodies in serologically characterized sera from patients with Lyme-like symptoms to evaluate their diagnostic potential. RESULTS: Results showed that chimeras vary in their immuno-reactivity when tested with human sera. Compared with the results obtained from a two-tier test, the application of recombinant multi-epitope chimeric proteins as diagnosis antigens, produced fair agreement in the case of A/C-2 (0.20
Source: Annals of Agricultural and Environmental Medicine : AAEM - Category: Environmental Health Authors: Tags: Ann Agric Environ Med Source Type: research

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This study can’t contribute to the debate over whether liberals or conservatives are more likely to commit such errors, the researchers write, because the stimuli weren’t constructed to be equally polarising to the two “sides” (though some prior research suggest both tribes are equally vulnerable). It also doesn’t tell us what can be done about this sort of ill-formed reasoning. Though on that front, at least, Gampa, Wojcik, and their colleagues do have some ideas: “A takeaway from this research… may be that reasoners should strive to be epistemologically humble. If logical reason...
Source: BPS RESEARCH DIGEST - Category: Psychiatry & Psychology Authors: Tags: Cognition Political Thought Source Type: blogs
Conclusion: The level of parasite contamination is high and consumption of raw vegetables procured from these markets carry a high risk of parasite infection. Washing vegetables twice with saline was not effective for parasite removal. Improved approaches for washing vegetables before consumption are needed. Funding: Ensign College-Students Research Award Program of Bob and Lynette Gay. PMID: 30662081 [PubMed - in process]
Source: Ghana Medical Journal - Category: African Health Tags: Ghana Med J Source Type: research
Conclusion: Urinary survivin is highly sensitive, specific and predictive of bladder carcinoma in our environment. The marker is associated with false positive results in patients with prostate cancer. Funding: By authors. PMID: 30662078 [PubMed - in process]
Source: Ghana Medical Journal - Category: African Health Tags: Ghana Med J Source Type: research
Steven C. Ricke, Kristina M. Feye, W. Evan Chaney, Zhaohao Shi, Hilary Pavlidis, Yichao Yang
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Authors: Dundar D, Duymaz Z, Genc S, Er DK, ─░rvem A, Kandemir N Abstract The aim of the study is to determine in-vitro effects of imipenem-tigecycline, imipenem-colistin and tigecycline-colistin against carbapenem-resistant Enterobacteriaceae (CRE) isolates. A total of 25 CRE isolates were included to the study. The minimum inhibition concentrations of imipenem, colistin-sulphate and tigecycline were determined with broth dilution method. Synergistic effects of imipenem-tigecycline, imipenem-colistin and tigecycline-colistin were investigated by microdilution checkerboard technique. All of the isolates were resist...
Source: Journal of Chemotherapy - Category: Cancer & Oncology Tags: J Chemother Source Type: research
Authors: Pezeshki Najafabadi M, Dagoohian A, Rajaie S, Zarkesh-Esfahani SH, Edalati M Abstract Urinary tract infections (UTIs) are considered the most common community-acquired infections worldwide, which have possible complications along with significant economic impact on national healthcare systems. The aim of this study was to identify the most common causes of significant bacteriuria and to assess their antimicrobial resistance pattern in the Isfahan province of Iran. In this cross-sectional study, 11,678 urine samples of the patients referred to Mahdieh Medical Diagnostic Centre Charity were examined over a p...
Source: Journal of Chemotherapy - Category: Cancer & Oncology Tags: J Chemother Source Type: research
This study aimed to investigate the in vitro antimicrobial properties of second-generation ceragenins, CSA-142 and CSA-192, and compare them to CSA-13. The antimicrobial activities of CSA-13, CSA-142 and CSA-192 were studied against 20 strains of Escherichia coli, Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans. Time-kill curve methods were performed to determine the bactericidal and fungicidal activities of the ceragenins against three strains of each microorganism. Overall, CSA-13 showed the lowest MIC values, but also CSA-142 and CSA-192 had good activities against tested micr...
Source: Journal of Chemotherapy - Category: Cancer & Oncology Tags: J Chemother Source Type: research
Publication date: Available online 22 January 2019Source: Materials Science and Engineering: CAuthor(s): Hakimeh Teymourinia, Masoud Salavati-Niasari, Omid Amiri, Fatemeh YazdianAbstractTiO2/Sb2S3/GQDs nanocomposite was prepared by solvothermal method to study its antibacterial properties. XRD, SEM, EDX, FT-IR and TEM were used to characterize prepared nanomaterials. To evaluate effect of graphene quantum dots (GQD) on the antibacterial activity of final products, antibacterial activity of TiO2, TiO2/GQDs, Sb2S3, Sb2S3/GQDs were compared with TiO2/Sb2S3/GQDs. TiO2/Sb2S3/GQDs shows the highest antibacterial activity against...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 22 January 2019Source: Materials Science and Engineering: CAuthor(s): Xiaochao Ji, Xiaoying Li, Yangchun Dong, Rachel Sammons, Linhai Tian, Helong Yu, Wei Zhang, Hanshan DongAbstractSilver is considered promising in medical devices to prevent infection due to its excellent properties of broad antibacterial spectrum and persistent antibacterial activity. Herein, silver impregnated functionally graded composite surfaces have been developed by a novel duplex plasma deposition technique, which combines the double glow sputtering process and active screen plasma nitriding process. The composit...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: Available online 21 January 2019Source: Small Ruminant ResearchAuthor(s): Nirmal Chacko, Sanchay Kumar Biswas, Nihar Nalini Mohanty, Karam Chand, Awadh Bihari Pandey, Bimalendu Mondal, Sathish Bhadravati ShivachandraAbstractBluetongue viral antibody detection assays are important for routine disease diagnosis and sero-surveillance in susceptible host’s especially small ruminants. In the present study, a partial NS2 gene encoding for two non-structural protein-2 fragments; N-terminus (1M-A177 aa) and C-terminus (178P-V354 aa) of BTV-23, were cloned separately, expressed and purified as recombinant NS...
Source: Small Ruminant Research - Category: Zoology Source Type: research
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