Detection of Escherichia coli producing CTX-M-1-group extended-spectrum β-lactamases from pigs in Aichi prefecture, Japan, between 2015 and 2016.

Detection of Escherichia coli producing CTX-M-1-group extended-spectrum β-lactamases from pigs in Aichi prefecture, Japan, between 2015 and 2016. Jpn J Infect Dis. 2017 Dec 26;: Authors: Norizuki C, Kawamura K, Wachino JI, Suzuki M, Nagano N, Kondo T, Arakawa Y Abstract We investigated the prevalence and characteristics of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from Japanese pigs. A total of 345 pig fecal specimens were collected from 30 farms in Aichi prefecture, Japan, between June 2015 and April 2016, and 22 unique ESBL-producing E. coli were isolated from 16 samples of eight farms. The ESBL types included CTX-M-15 (54.5%), CTX-M-55 (27.2%), CTX-M-3 (0.9%), and CTX-M-14 (0.9%). The predominant plasmid replicon type was IncN, and they carried blaCTX-M-55. Nine STs, including ST117, ST1706, ST38, and ST10 were detected in ESBL-producers, but no B2-O25-ST131 was found. ESBL producers were highly resistant to cefotaxime, ceftiofur, and tetracycline but susceptible to imipenem, amikacin, and fosfomycin, although two ST354 isolates showed resistance to ciprofloxacin. All 11 chloramphenicol-resistant isolates, including ST117 (n = 6) and ST38 (n = 3), harbored floR, and two fosfomycin-resistant ST38 isolates harbored fosA3. Our results suggested that pigs may not act as a direct reservoir in the transmission of ESBL genes in humans. However, ST117 E. coli carrying IncN-type plasmids mediating blaCTX-M-...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research