Comparison of three molecular subtyping methods among O157 and non-O157 Shiga toxin-producing Escherichia coli isolates from Japanese cattle.

In this study, we compared three molecular subtyping methods, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and multiple-locus variable number of tandem repeat analysis (MLVA), using O157 and non-O157 STEC isolated from Japanese beef cattle. A total of 73 STEC isolates belonging to 9 O-serogroups were used. By employing three molecular subtyping methods, the strains were divided into 9 MLST sequence types (STs), 23 PFGE types and 26 MLVA types. The STEC classification by O-serogrouping and MLST was almost identical. Furthermore, PFGE and MLVA could systematically classify STEC of same serotypes and STs. MLVA and PFGE were found to be highly efficient subtyping methods following O-serogrouping, for the classification of not only O157, but also non-O157 STEC isolates in outbreak investigation. PMID: 29279448 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research