Prevalence and Genotype Distribution of Chlamydia trachomatis in Urine among Men Attending STD Clinics in Guangdong Province, China, 2016.

This study aimed to investigate the prevalence and molecular epidemiology of CT infection by examining urine samples among MSCs from different geographic areas of Guangdong province, China. A cross-sectional study was conducted among MSCs from ten HIV sentinel surveillance sites in Guangdong province. CT DNA in men urine samples were extracted and detected by using the Roche cobas® 4800 CT/NG. The ompA genes were amplified by nested polymerase chain reaction (PCR) and sequenced. The leukocyte esterase test was performed by routine urine analysis at local clinics. Of the 1903 samples, 163(8.6%, 95%CI3.8- 16.3%) were found to be positive for CT. The highest prevalence (10.5%) of CT infection was observed among participants aged 21-30. One hundred and thirty CT positive specimens (79.8%, 130/163) were successfully genotyped by nested PCR, resulting in eight genotypes. The most prevalent genotypes were D, E, F, and J, with proportions of 20.8%, 20.0%, 17.7%, and 16.9%, respectively. There was no significant difference in geographic area, leukocyte esterase test and genotype distribution. Promotion of detection and molecular epidemiology research is needed for effective and comprehensive prevention and control programs. PMID: 29279452 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research