Evaluation of the FilmArray Blood Culture Identification Panel in Detecting Pathogenic Microorganisms from Positive Blood Cultures: the First Clinical Report in Japan.

Evaluation of the FilmArray Blood Culture Identification Panel in Detecting Pathogenic Microorganisms from Positive Blood Cultures: the First Clinical Report in Japan. Jpn J Infect Dis. 2017 Dec 26;: Authors: Saito K, Endo S, Katsumi M, Ishizawa C, Fujikawa Y, Inomata S, Toyokawa M, Kaku M Abstract   The FilmArray (FA) is a multiplex polymerase chain reaction (PCR)-based desktop microbial detection system. The Blood Culture Identification (BCID) panel is an adaptable panel for the FA, which diagnoses sepsis or/and systemic infections by detecting 14 bacterial species, 4 bacterial genera, 1 bacterial family, 5 yeast species, and 3 antimicrobial resistance genes (mecA, KPC, and vanA/B) from positive blood cultures within 1 hour. We retrospectively evaluated the FA-BCID panel, using 54 positive blood cultures, from which 57 bacteria and 3 yeasts were identified through matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. The FA-BCID panel revealed 59 microorganisms from 53 samples, which was similar the results of MALDI-TOF MS analysis; however, 1 bacterium from 1 sample was not detected. In addition, MecA genes were detected in 12 Staphylococcus species, which all displayed methicillin resistance on susceptibility testing, while KPC and vanA/B genes were not detected, which was consistent with the results of antimicrobial susceptibility testing. Although more information on antimicrobial resistance...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research

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