Elastin-Like Polypeptide incorporated thermally sensitive liposome improve antibiotic therapy against Staphyloccous aureus in vitro.

Elastin-Like Polypeptide incorporated thermally sensitive liposome improve antibiotic therapy against Staphyloccous aureus in vitro. Int J Hyperthermia. 2017 Dec 26;:1-22 Authors: Nigatu AS, Ashar H, Sethuraman SN, Wardlow R, Maples D, Malayer J, Ranjan A Abstract Musculoskeletal infections caused by bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa in children and adults can lead to adverse outcomes including a need for extensive surgical debridement and limb amputation. To enable targeted antimicrobial release in infected tissues, the objective of this study was to design and investigate novel Elastin-Like Polypeptide (ELP) based thermally-sensitive liposomes in vitro. ELP biopolymers can change their phase behavior at higher temperatures. We hypothesized that ELP-TSL will improve therapeutic efficacy by releasing antimicrobial payloads locally at higher temperatures (≥39°C). ELP-TSL library were formulated by varying cholesterol and phospholipid composition by the thin film and extrusion method. A broad-spectrum antimicrobial (Ciprofloxacin) was encapsulated inside the liposomes by the ammonium sulfate gradient method. Cipro release from ELP-TSLs was assessed in physiological buffers containing ∼25% serum by fluorescence spectroscopy, and efficacy against Staphylococcus aureus and Pseudomonas aeruginosa was assessed by disc diffusion and planktonic assay. Active loading of Cipro achieved an encapsulation effic...
Source: International Journal of Hyperthermia - Category: Internal Medicine Tags: Int J Hyperthermia Source Type: research