Heterogeneous expression of Chlamydia pneumoniae antigen candidates and high-level soluble expression of its inclusion membrane proteins in Escherichia coli

In this study, seven antigen candidates fromC. pneumoniae TW-183 (OmpH, Omp11, Eno, ArtJ, IncA, IncB and IncC) were screenedin silico genome. Immunogenicity of the heterogeneously overexpressed antigen candidates in E. coli BL21 (DE3) was investigated by Western blot analysis using in sera fromC. pneumoniae infected individuals. The soluble forms of three inclusion membrane proteins (IncA, IncB and IncC), carrying unique bilobal hydrophobic domains, were also successfully expressed inE. coli BL21 (DE3) through truncation of their membrane bound transmembrane domains.
Source: Molecular and Cellular Toxicology - Category: Cytology Source Type: research