Prevalence of Listeria monocytogenes in cerebrospinal fluid obtained from hospitalized patients

AbstractThe aim of the present study was to determine the occurrence ofListeria monocytogenes in cerebrospinal fluid (CSF) samples obtained from patients with meningitis symptoms. A total of 102 CSF samples which were referred for bacterial culture to microbiology laboratory in Mashhad ’s Hospital were included in this study. All of the samples were cultured and positive ones confirmed by multiplex-PCR. Molecular serotyping and virulence characterization of isolates were performed. Random amplified polymorphic DNA-PCR using three set of primers was performed for evaluation of ge netic diversity of these isolates. Five out of 102 samples were positive forL. monocytogenes. Three different serotypes were detected, and all of the isolates were potentially pathogenic. Low genetic diversity was determined among isolatedL. monocytogenes.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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Listeria monocytogenes meningitis in Danish children 1 month to 17 years from 2000 to 2017 was identified and patient files reviewed. There were 5 cases, equaling an annual incidence of 0.024 per 100,000 children or 0.014 when excluding 2 immunodeficient children. Even in a country with a high general incidence of listeriosis, Listeria meningitis is a rare event in healthy children.
Source: The Pediatric Infectious Disease Journal - Category: Infectious Diseases Tags: Brief Reports Source Type: research
Publication date: Available online 22 August 2019Source: Journal of Clinical NeuroscienceAuthor(s): Shih-Ying Chen, Jun-Jun Lee, Chun-Chih Chien, Wan-Chen Tsai, Cheng-Hsien Lu, Wen-Neng Chang, Chia-Yi LienAbstractThe clinical characteristics and therapeutic outcomes of adult Listeria monocytogenes meningitis are not commonly examined in isolation in the literature. During a study period of 19 years (2000–2018), 366 patients with culture-proven adult bacterial meningitis (ABM) were identified in the author’s hospital (264 patients in 2000–2010 and 102 patients in 2011–2018). Of the 366 ABM patients...
Source: Journal of Clinical Neuroscience - Category: Neuroscience Source Type: research
In this study, occurrence and contribution of this plasmid to BC tolerance was examined using PCR, plasmid curing and transformation, RT-qPCR and proteome analysis, respectively. Furthermore, the substrate specificity of the pLMST6 associated EmrC efflux pump and the impact of the plasmid on L. monocytogenes virulence was investigated. pLMST6 was detected in 7 (1.6%) of 439 L. monocytogenes strains isolated from different sources. A phenotypic role of this plasmid in conferring increased BC tolerance was confirmed by showing that plasmid cure increases BC susceptibility whereas plasmid complementation and transformation in...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusions Listeriosis in pregnancy can result in severe adverse maternal, fetal, and neonatal outcomes including miscarriage, preterm labor, fetal death, and neonatal meningitis and sepsis. Early treatment has been shown to improve fetal and neonatal outcomes; therefore, prevention with education and early diagnosis prompting treatment will improve overall outcomes. Relevance The incidence of listeriosis is expected to rise in the coming years due to changes in the US population, with increasing numbers of older Americans and Hispanic individuals, both of whom are at higher risk. Pregnant women contract listeriosis ...
Source: Obstetrical and Gynecological Survey - Category: OBGYN Tags: CME ARTICLES Source Type: research
CONCLUSIONS: Our two cases demonstrate that the listeriosis outbreak should change the way we view bacterial meningitis in SA: according to the National Institute for Communicable Diseases, empirical treatment for meningitis should include ampicillin and gentamicin in all adult patients with features of meningitis. There may be a need for an updated meningitis treatment guideline in SA. PMID: 31131793 [PubMed - in process]
Source: South African Medical Journal - Category: African Health Tags: S Afr Med J Source Type: research
art P Abstract Listeria monocytogenes is a food-borne bacterial pathogen that is responsible for listeriosis, a disease characterized by occasional febrile gastroenteritis in immunocompetent individuals, abortions in pregnant women, meningitis in the newborn and fatal bacteraemia in immunocompromised individuals or the elderly. The ability of L. monocytogenes to produce disease is intimately associated with its potential to traverse several human barriers (including the intestinal, placental and blood/brain barriers), to promote its internalization within diverse populations of epithelial cells and to proliferate ...
Source: Microbiology - Category: Microbiology Authors: Tags: Microbiology Source Type: research
Abstract Listeria monocytogenes is a gram-positive, facultative anaerobic, motile bacillus widely spread in the environment. However, clinical infection in humans is uncommon. The route of infection is often through ingestion of raw vegetables, meat and unpasteurised dairy products. Listeria is an uncommon cause of meningitis in immunocompetent individuals, but it should be considered strongly in neonates and the elderly, and it should also be considered in those with impaired cell-mediated immunity. 1 , 2 Only a few case reports of invasive listeriosis in humans have been reported from India. 3 - 8 In our case, a...
Source: Tropical Doctor - Category: Tropical Medicine Authors: Tags: Trop Doct Source Type: research
We present a case of a 23 month-old boy presenting with fever, irritability and diarrhea who subsequently developed symptoms of photophobia and lethargy. Cerebrospinal fluid culture grew Listeria monocytogenes. Immunology investigations were normal. This patient had a complete and uncomplicated recovery. Listeria meningitis is a rare presentation in immunocompetent children, but should be considered in the setting of diarrhea, failure to respond to cephalosporin therapy, or suspected immunodeficiency. PMID: 31411392 [PubMed - in process]
Source: Ir Med J - Category: General Medicine Authors: Tags: Ir Med J Source Type: research
Feixia Zheng1*, Xiuyun Ye1, Xulai Shi1, Zhongdong Lin1, Zuqin Yang1 and Longxiang Jiang2* 1Department of Pediatrics, The Second Affiliated Hospital &Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China 2Department of Respiratory Medicine, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou, China Background: Hyponatremia has frequently been described as a common complication associated with bacterial meningitis, though its frequency and clinical course in children with bacterial meningitis are unclear. The present study aimed to investigate the frequen...
Source: Frontiers in Neurology - Category: Neurology Source Type: research
Purpose of review Community-acquired bacterial meningitis continues to occur and be associated with significant morbidity and mortality despite the availability of effective conjugate vaccines for the three most important meningeal pathogens. Recent findings Indications for cranial imaging in suspected bacterial meningitis varies significantly between guidelines. Cranial imaging is of no clinical utility in those patients without indications and fosters delays in performing a lumbar puncture. Delaying lumbar puncture is associated with increased costs in both adults and children with meningitis and previous antibiotic...
Source: Current Opinion in Infectious Diseases - Category: Infectious Diseases Tags: CNS INFECTIONS: Edited by Adarsh Bhimraj Source Type: research
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