Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS).

Conclusion: In this study, we developed a reliable FACS-based procedure using ethanol as a fixative that would support accurate isolation of limbal epithelial progenitor subpopulations along with RNA yield and quality sufficient to enable deep transcriptomic profiling. PMID: 29255379 [PubMed]

https://www.ncbi.nlm.nih.gov/pubmed/29255379?dopt=Abstract

Source: Biological Procedures Online - December 21, 2017 Category: Molecular Biology Authors: Liu L, Nielsen FM, Riis SE, Emmersen J, Fink T, Hjortdal JØ, Bath C, Zachar V Tags: Biol Proced Online Source Type: research

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