Cortical and spinal conditioned media modify the inward ion currents AND excitability and PROMOTE DIFFERENTIATION of human striatal primordium.

Publication date: Available online 20 December 2017 Source:Journal of Chemical Neuroanatomy Author(s): Eglantina Idrizaj, Erica Sarchielli, Annamaria Morelli, Rachele Garella, Maria Caterina Baccari, Pasquale Gallina, Gabriella Barbara Vannelli, Fabio Francini, Roberta Squecco Human striatal precursor cells (HSPs) isolated from ganglionic eminence may differentiate in electrophysiologically functional excitable neuron-like cells and a number of endogenous molecules such as hormones, neurotransmitters or growth factors can actually regulate neuronal growing and differentiation. The purpose of this research was to assess, by electrophysiological and immunocytochemical analysis, if the type of culture medium could specifically impact on the neuronal differentiation potential of HSPs. Accordingly, HSPs were maintained in different inductive media such as cortical and spinal cord conditioned media, and we estimated the possible changes in the main ion currents, neuronal excitability and expression of neuronal markers indicative of neuronal differentiation. Our results have shown that 36 h exposure to each of the conditioned media, with their blend of autocrine and paracrine growth factors, was able to modify significantly the electrophysiological membrane properties and the functional expression of inward ionic currents in selected neuronal HSPs. Moreover, although both types of conditioned media determined neuronal maturation (increased neuritogenesis and increased e...
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research