Interactions of Salmonella enterica sv. Typhimurium and Pectobacterium carotovorum within a Tomato Soft Rot.

Interactions of Salmonella enterica sv. Typhimurium and Pectobacterium carotovorum within a Tomato Soft Rot. Appl Environ Microbiol. 2017 Dec 15;: Authors: George AS, Cox CE, Desai P, Porwolik S, Chu W, de Moraes MH, McClelland M, Brandl MT, Teplitski M Abstract The human pathogen Salmonella is remarkably adaptable, and this trait allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amidst the native microbiota remains poorly understood. Here, we aimed to uncover the mechanisms that enable Salmonella enterica sv. Typhimurium to benefit from the degradation of plant tissue by a soft-rot plant pathogen Pectobacterium carotovorum. The hypothesis that in the soft rot, the liberation of starch (not utilized by P. carotovorum) makes this polymer available to Salmonella, thus allowing it to colonize soft rots was tested first and proven null. To identify the functions involved in the Salmonella soft rot colonization, we carried out transposon insertion sequencing coupled with the phenotypic characterization of the mutants. The data indicate that Salmonella experiences a metabolic shift in response to the changes in the environment brought on by Pectobacterium and likely coordinated by the csrBC small regulatory RNA. While csrBC and flhD appear to be of importance in the soft rot, the global two-component system encoded by barA/sirA (which controls csrBC an...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research