Evaluation of Diagnostic Utility of a High-Risk Human Papillomavirus PCR Test on Formalin Fixed, Paraffin-Embedded Head and Neck Tumor Tissues

The increasing prevalence of high-risk human papillomavirus (HR-HPV) –associated head and neck squamous cell carcinoma (HNSCC) has prompted strong clinical demands for detecting HR-HPV directly in the tumor. Although p16 immunohistochemistry (IHC) has been the standard testing method, it has limitations including false positivity, lack of sensitivity in low tumor c ell samples such as fine needle aspirate (FNA), and its subjectivity. We developed a modified method based on the Roche Cobas 4800 HR-HPV PCR assay and evaluated the performance characteristics and the diagnostic utility of this assay for direct HR-HPV detection in the HNSCC samples.
Source: Journal of Molecular Diagnostics - Category: Pathology Authors: Tags: Regular Article Source Type: research