Rapid Colorimetric Detection of Zika Virus from Serum and Urine Specimens by RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification).

The Zika virus (ZIKV) can be passed from a pregnant woman to her fetus. Resulting infection by this virus can cause early miscarriage and a pattern of severe birth defects in fetuses and infants. Therefore, a rapid diagnostic assay that can be performed throughout pregnancy in a clinical setting is vital for prenatal care of women living in areas where this virus may be transmitted.CDC has developed a RT-LAMP assay for the rapid screening of ZIKV RNA from urine and serum. As few as 10 copies of ZIKV RNA per microliter can be detected using this assay, showing that it is highly sensitive. It was also shown to be highly specific for ZIKV RNA when tested against a panel of urine samples spiked with other flaviviruses such as dengue virus sub-types 1-4, West Nile virus, St. Louis encephalitis virus, and chikungunya virus, most viruses that circulate in the same geographic regions as ZIKV. This assay offers a rapid, reliable, sensitive, and specific means to detect ZIKV that can be performed in a clinical point-of-care (POC) or field setting with minimal equipment and technological expertise. The assay is fast to set up and perform (total time 1 hour). Additionally, it is approximately as sensitive and specific as RT-PCR detection methods. Thus far, 178 clinical samples collected from the 2015/2016 Zika virus outbreak were tested using this assay. CDC is currently working with a group to test a prototype point of care (POC) test in a clinical setting and develop a POC.IC: CDC...
Source: NIH OTT Licensing Opportunities - Category: Research Authors: Source Type: research

Related Links:

HPV DNA detection in urine samples of women: "An efficacious and accurate alternative to cervical samples?" Expert Rev Anti Infect Ther. 2019 Sep 13;: Authors: Pattyn J, Van Keer S, Téblick L, Van Damme P, Vorsters A Abstract Cervical cancers are caused by persistent human papillomavirus (HPV) infections [1]. To date, HPV DNA testing using clinician-obtained cervical cells is the reference for identifying cervical infection. However, great interest has been shown for the use of self-sampling methods to detect HPV [2]. One method for self-sampling to be considered is the collection of f...
Source: Expert Review of Anti-Infective Therapy - Category: Infectious Diseases Tags: Expert Rev Anti Infect Ther Source Type: research
ConclusionActual body weight and bone marrow involvement may be utilized as potential predictive indicators of R-IRRs. Further study is needed to validate these indicators and determine appropriate utilization in practice.
Source: Clinical Lymphoma Myeloma and Leukemia - Category: Cancer & Oncology Source Type: research
Conclusion: Specific contexts appeared to be associated with problematic drinking for both sexes. Among both men and women, "social drinking" was associated with problematic drinking. Both "home drinking" (men) and "daytime drinking" (women) contexts, were associated with problematic drinking and depressive symptoms. Targeted alcohol-focused interventions need to address co-occurring mental health issues. PMID: 31519127 [PubMed - as supplied by publisher]
Source: Substance Use and Misuse - Category: Addiction Tags: Subst Use Misuse Source Type: research
Conclusions: Reduced expression of ER stress markers in trophoblast cells against increased VEGF highlighted a new window to explore prospective drugs that can be designed to modulate the activities of various ER stress sensors in order to alleviate ER stress in pregnant women with preeclampsia. PMID: 31517301 [PubMed]
Source: Reproductive Biology - Category: Reproduction Medicine Authors: Tags: Eur J Obstet Gynecol Reprod Biol X Source Type: research
Abstract Objective: The aims of this study were to determine the prevalence of human papillomavirus (HPV) infection in women treated for infertility and oocyte donors, and to investigate the possible influence of HPV infection on reproductive outcomes. Study design: In this observational laboratory-based study, cervical swabs were collected from oocyte donors (n = 207), and women treated for infertility (n = 945) and analysed for the presence of high-risk HPV (hrHPV) genotypes using the cobas® 4800 HPV Test and PapilloCheck® HPV-Screening. Associations between hrHPV positiv...
Source: Reproductive Biology - Category: Reproduction Medicine Authors: Tags: Eur J Obstet Gynecol Reprod Biol X Source Type: research
Authors: Friedman SH, Tamburello AC, Kaempf A, Hall RCW Abstract Prescribing for women in jails and prisons requires special consideration and should be informed by extant evidence. Incarcerated women have higher rates of mental illness than both females in the community and incarcerated men. Medication administration concerns that may disproportionately affect female prisoners include drug-drug interactions with contraceptives, intermittent dosing schedules, and concerns about metabolic side effects. Further, pregnancy, lactation, and menopause may all affect medication choices. Incarcerated women frequently have ...
Source: Journal of the American Academy of Psychiatry and the Law - Category: Medical Law Tags: J Am Acad Psychiatry Law Source Type: research
MYCOBACTERIUM CHIMAERA INFECTIONS AFTER CARDIOVASCULAR SURGERY: LESSONS FROM A GLOBAL OUTBREAK. Trans Am Clin Climatol Assoc. 2019;130:136-144 Authors: Diekema DJ Abstract A global outbreak of invasive Mycobacterium chimaera infections has been associated with exposure to certain heater-cooler devices (HCDs) used during cardiopulmonary bypass. Outbreak investigations have shown that these HCDs harbor M. chimaera in water circuits and generate bio-aerosols in the operating room, leading to airborne transmission to patients during surgery. Whole genome sequencing data support a common-source outbreak ori...
Source: Transactions of the American Clinical and Climatological Association - Category: General Medicine Tags: Trans Am Clin Climatol Assoc Source Type: research
THE GORDON WILSON LECTURE: THE HEPATITIS C VIRUS: FROM HIPPOCRATES TO CURE. Trans Am Clin Climatol Assoc. 2019;130:104-118 Authors: Alter HJ Abstract The modern age of viral hepatitis began in the early 1960s with the serendipitous discovery of the Australia antigen, a protein that was later shown to represent the envelope of the hepatitis B virus leading to its designation as the hepatitis B surface antigen. This was the first marker for any hepatitis virus and became not only a diagnostic assay, but also a mandatory blood donor screening test and the basis for the first generation hepatitis B vaccine...
Source: Transactions of the American Clinical and Climatological Association - Category: General Medicine Tags: Trans Am Clin Climatol Assoc Source Type: research
CONCLUSION: Administration of pirimiphos-methyl resulted in a reduced implantation rate due to decreased uterine receptivity caused by an imbalance in the level of estradiol and progesterone and impaired reproductive outcome during pregnancy. PMID: 31517635 [PubMed - in process]
Source: Endocrine Regulations - Category: Endocrinology Tags: Endocr Regul Source Type: research
Conclusions Apart West Nile virus (78), thousands of congenital microcephaly cases, fetal brain tissue damage and neurological syndromes have been associated with ZIKV infection. Unfortunately, the epidemics of this mosquito born, and a relative stable virus is on a rise. Although congenital microcephaly is a rare disorder however, due to lack of standardized diagnostic test facilities, the incidence in the geographically widespread ZIKV epidemic regions is higher. Animals studies showed that ZIKV is a neurotropic virus. It directly targets the developing embryonic brain cells by inducing apoptosis, cell-cycle arrest, and...
Source: Frontiers in Neurology - Category: Neurology Source Type: research
More News: Chikungunya | Dengue Fever | Encephalitis | Genetics | Infectious Diseases | International Medicine & Public Health | Miscarriage | Outbreaks | Pregnancy | Research | Vaccines | West Nile Virus | Women | Zika Virus