Evaluation of VITEK 2 Compact and VITEK MS in the identification of coagulase-negative staphylococci isolated from blood cultures

Conclusion: Through the analysis between the two devices and based on scientific articles comparing the methods involved in the study, it was possible to observe the great biochemic al similarity among the coagulase-negative staphylococci species, this being the main limitation of the phenotypic method. The VITEK MS equipment showed high discrimination between species when compared to the gold standard method, besides being rapid and easy to use.RESUMO Introdu ção: Estafilococos coagulase-negativos (ECN) estão entre os principais colonizantes de pele e mucosas, atuando como comensais. Apesar de apresentarem baixa virulência, podem causar infecções graves, principalmente quando são utilizados dispositivos invasivos, como cateteres. Como têm grande capacidade de formar biofilme, conseguem atingir rapidamente a corrente sanguínea, o que pode levar à septicemia. Objetivo: O objetivo principal foi comparar a identificação dos ECN entre duas metodologias automatizadas: fenotípica (VITEK® 2 Compact, bioMérieux) e espectrometria de massa (MA LDI-TOF VITEK® MS, bioMérieux). Materiais e método: Foram analisados 133 isolados de ECN provenientes de amostras de hemoculturas, no período de setembro de 2015 a maio de 2016. Resultados: Todos os 133 isolados foram identificados no VITEK® 2 Compact como ECN, enquanto o VITEK MS não identi ficou sete amostras; ...
Source: Jornal Brasileiro de Patologia e Medicina Laboratorial - Category: Pathology Source Type: research

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Source: The Journal of Allergy and Clinical Immunology: In Practice - Category: Allergy & Immunology Source Type: research
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Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research
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Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
In this study we present the first crystal structure model for bone apatite based on the analysis of individual nanocrystals by high resolution transmission electron microscopy (HRTEM). Crystallographic image processing of the obtained HRTEM images from different projections indicates symmetry reduction with respect to P63/m stoichiometric apatites and the presence of threefold symmetry along the c axis. Based on HRTEM observations and the measured Ca/P = 2 ratio we propose a structural model with phosphate-to-carbonate substitution and O vacancies localized along c axis, which explains the observed loss of 63 screw ax...
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Source: Materials Science and Engineering: A - Category: Materials Science Source Type: research
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Source: Materials Letters - Category: Materials Science Source Type: research
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