Effects of shRNA ‐mediated Silencing of PSMA7 on Cell Proliferation and Vascular Endothelial Growth Factor Expression via the Ubiquitin‐Proteasome Pathway in Cervical Cancer

This study aims to evaluate the effects of PSMA7 silencing on cervical cancer (CC) cell proliferation and vascular endothelial growth factor (VEGF) expression through the ubiquitin‐proteasome (UPP) pathway. CC tissue (n = 43) and normal tissues (n = 27) were first collected from patients. Human CC cell line (SiHa) and human normal cervical epithelial cells (H8) were obtained and classified into the normal, blank, negative control (NC), PSMA7‐shRNA1 and PSMA7‐shRNA2 groups. Hybridization in situ was used to detect the expressions of wild‐type and mutant p53 proteins. Immunofluorescence assay was carried out to test the activity of 20S proteasomes. RT‐qPCR and Western blotting were both performed to determine the expressions of PSMA7, ubiquitin, P27, P53 and VEGF in sample tissues and cells. MTT assay was used analyze cell proliferation rates, and flow cytometry to analyze cell cycle and apoptotic rate. Compared with normal tissue, CC tissues had increased expression levels of PSMA7, ubiquitin, p53, VEGF as well as increased the activity of 20S proteasomes, but exhibited a decrease in p27 expression. Compared with the blank and NC groups, the PSMA7‐shRNA1 and PSMA7‐shRNA2 groups all had decreased expression levels of PSMA7, ubiquitin, p53 and VEGF as well as decreased cell proliferation, 20S proteasomes activity and cell number in the S phase, increased p27 expression, cell apoptosis and cell number in the G0/G1 phase. Our study demonstrates that PSMA7 sil...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research