Molecular detection of Brucella spp in goat and sheep milk samples from apparently healthy and infected animals

This study set to assess the PCR assay for the molecular recognition ofBrucella DNA in goats and sheep unpasteurized milk samples in the southeast region of Iran and to determine the frequency distribution of seasonal outbreaks. Eight hundred samples of raw milk from 722 apparently healthy animals and 78 infected animals with a history of abortion were collected from January 2015 to June 2016. In our PCR, a specific genome region, the IS711 element, was targeted to amplifyBrucella DNA, and the frequency distribution of seasonal outbreaks was determined. PCR assay amplifiedBrucella DNA in 10.85% goats and 5.45% sheep milk samples (CI  = 95). Among samples with abortion history, the disease occurrence rate was 67.94% but among those without abortion history, it was 2.21%. The most frequent rates of brucellosis were seen in the summer (n = 33), and the lowest rate occurred in the winter (n = 3). The results indicated a significantly higher prevalence (p 
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research

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AbstractDromedary, or one-humped, camelsCamelus dromedarius are an almost exclusively domesticated species that are common in arid areas as both beasts of burden and production animals for meat and milk. Currently, there are approximately 30 million dromedary camels, with highest numbers in Africa and the Middle East. The hardiness of camels in arid regions has made humans more dependent on them, especially as a stable protein source. Camels also carry and may transmit disease-causing agents to humans and other animals. The ability for camels to act as a point source or vector for disease is a concern due to increasing hum...
Source: EcoHealth - Category: Environmental Health Source Type: research
Authors: Cama BAV, Ceccarelli M, Venanzi Rullo E, Ferraiolo F, Paolucci IA, Maranto D, Mondello P, Lo Presti Costantino MR, Marano F, D'Andrea G, Di Marco V, Puglisi G, Valenzise M, D'Angelo G, Mondello L, Strano G, Condorelli F, Spicola D, Nunnari G, Pellicanò GF Abstract Brucellosis is one of the most common zoonoses in the world, especially in Southern Italy, where many cases are still recorded every year. 128 cases of brucellosis were observed in Messina (Sicily) in 2016, representing a tenfold increase in the number of cases of brucellosis expected. The aim of this multicenter retrospective study was to...
Source: New Microbiologica - Category: Microbiology Tags: New Microbiol Source Type: research
Abstract Zoonoses such as Brucella, Mycobacterium bovis, Campylobacter, Salmonella, Escherichia coli, Listeria, etc. are spread by ingestion of contaminated unpasteurised milk products (from infected cattle) and result in infectious outbreaks, hospitalisations and mortality. In Southeast Asian countries, raw goat's milk is sometimes used for dengue infection-associated thrombocytopenia; however, evidence to support this sociocultural practice is lacking. A peak incidence of brucellosis cases may occur after a dengue season in communities where this practice is prevalent. PMID: 30895885 [PubMed - as supplied by publisher]
Source: Tropical Doctor - Category: Tropical Medicine Authors: Tags: Trop Doct Source Type: research
Publication date: Available online 3 December 2018Source: Journal of Microbiological MethodsAuthor(s): Triveni Kalleshamurthy, Chaithra Yaranna, Ranjitha Shekar, Krithiga Natesan, Swati Sahay, Bibek Ranjan Shome, Habibur Rahman, Sukhadeo B. Barbuddhe, Nagendra Nath Barman, Sailendra Kumar Das, Rajeswari ShomeAbstractBrucellosis in pigs, caused by the bacterium Brucella suis, is an important zoonotic infection. In the present study, fluorescence polarization assay (FPA) was standardized and compared with indirect enzyme linked immunosorbent assay (iELISA) and competitive ELISA (cELISA) for diagnosis of porcine brucellosis. ...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research
by Jennifer Mesureur, Sandrine Arend, B éatrice Cellière, Priscillia Courault, Pierre-Jean Cotte-Pattat, Heather Totty, Parampal Deol, Virginie Mick, Victoria Girard, Joanne Touchberry, Vanessa Burrowes, Jean-Philippe Lavigne, David O’Callaghan, Valérie Monnin, Anne KerielBrucella are highly infectious bacterial pathogens responsible for a severely debilitating zoonosis called brucellosis. Half of the human population worldwide is considered to live at risk of exposure, mostly in the poorest rural areas of the world. Prompt diagnosis of brucellosis is essential to prevent complications and to con...
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
Authors: Cama BAV, Ceccarelli M, Venanzi Rullo E, Ferraiolo F, Paolucci IA, Maranto D, Mondello P, Lo Presti Costantino MR, Marano F, D'Andrea G, Di Marco V, Puglisi G, Valenzise M, D'Angelo G, Mondello L, Strano G, Condorelli F, Spicola D, Nunnari G, Pellicanò GF Abstract Brucellosis is one of the most common zoonoses in the world, especially in Southern Italy, where many cases are still recorded every year. 128 cases of brucellosis were observed in Messina (Sicily) in 2016, representing a tenfold increase in the number of cases of brucellosis expected. The aim of this multicenter retrospective study was to...
Source: New Microbiologica - Category: Microbiology Tags: New Microbiol Source Type: research
ć Ž Abstract The most recent data on the incidence of brucellosis in Southeast Europe prove the persistence of this zoonosis in the area, regardless of constant efforts at controlling it as one of the most dangerous zoonoses. Forty-three Brucella melitensis strains were collected from cattle, sheep, goats and humans from Croatia as well as Bosnia and Herzegovina between 2009 and 2015. The strains were identified and genotyped in order to determine their epidemiological background. Standard biotyping methods and Bruce-ladder were used to identify the strains. Genotyping was done using multilocus variable number t...
Source: Acta Veterinaria Hungarica - Category: Veterinary Research Authors: Tags: Acta Vet Hung Source Type: research
Publication date: Available online 11 September 2017 Source:Small Ruminant Research Author(s): Renée de Cremoux, Kristel Gache, Elodie Rousset, Carole Sala, Soline Hosteing, Philippe Nicollet, Frédéric Lars, Raphaël Guatteo, Françoise Dion, Didier Calavas, Anne Bronner, Jean-Baptiste Perrin, Anne Touratier Multiple challenges have to be faced both for animal and public health in the fields of infectious abortion surveillance especially when zoonotic diseases are concerned: ensuring early detection of brucellosis, improving outbreak surveillance as well as animal health management. Thus, a ...
Source: Small Ruminant Research - Category: Zoology Source Type: research
Brucellosis, the most common zoonotic disease worldwide, represents a great threat to animal husbandry with the potential to cause enormous economic losses. Brucellosis has become a major public health problem in China, and the number of human brucellosis cases has increased dramatically in recent years. In order to evaluate different intervention strategies to curb brucellosis transmission in China, a novel mathematical model with a general indirect transmission incidence rate was presented. By comparing the results of three models using national human disease data and 11 provinces with high case numbers, the best fitted ...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Authors: Suvak B, Dulger AC, Suvak O, Yesilyurt AÖ, Gultepe B, Guducuoglu H Abstract INTRODUCTION: Hepatitis D virus (HDV) infection is a serious health problem leading to cirrhosis and hepatocellular carcinoma (HCC). Despite evidence that zoonotic infections are associated with end-stage liver disease, brucellosis in patients with delta hepatitis related to liver disease has not been well characterized. So, we examined this relationship using recent hospital-based data. MATERIAL AND METHODS: We analyzed data from 96 delta hepatitis patients (mean age: 52.5 ±12.8 years; 50 male; 52 cirrhotics) and 1...
Source: Archives of Medical Science - Category: Biomedical Science Tags: Arch Med Sci Source Type: research
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