Nanodesign of new self-assembling core-shell gellan-transfersomes loading baicalin and in vivo evaluation of repair response in skin

Publication date: Available online 15 December 2017 Source:Nanomedicine: Nanotechnology, Biology and Medicine Author(s): Maria Manconi, Maria Letizia Manca, Carla Caddeo, Donatella Valenti, Claudia Cencetti, Octavio Diez-Sales, Amparo Nacher, Silvia Mir-Palomo, Maria Carmen Terencio, Davide Demurtas, Juan Carmelo Gomez-Fernandez, Francisco José Aranda, Anna Maria Fadda, Pietro Matricardi Gellan nanohydrogel and phospholipid vesicles were combined to incorporate baicalin in new self-assembling core-shell gellan-transfersomes obtained by an easy, scalable method. The vesicles were small in size (~107nm) and monodispersed (P.I. ≤ 0.24), forming a viscous system (~ 24mPa/s) as compared to transfersomes (~ 1.6mPa/s), as confirmed by rheological studies. Gellan was anchored to the bilayer domains through cholesterol, and the polymer chains were distributed onto the outer surface of the bilayer, thus forming a core-shell structure, as suggested by SAXS analyses. The optimal carrier ability of core-shell gellan-transfersomes was established by the high deposition of baicalin in the skin (~ 11% in the whole skin), especially in the deeper tissue (~ 8% in the dermis). Moreover, their ability to improve baicalin efficacy in anti-inflammatory and skin repair tests was confirmed in vivo in mice, providing the complete skin restoration and inhibiting all the studied inflammatory markers. Graphical abstract
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research