Genome-Wide Profiling Reveals That Herbal Medicine Jinfukang-Induced Polyadenylation Alteration Is Involved in Anti-Lung Cancer Activity.

In this study, we investigated whether JFK exerts its antitumor effect by modulating APA patterns in lung cancer cells. We performed a genome-wide APA site profiling analysis in JFK treated lung cancer cells A549 with 3T-seq approach that we reported previously. Comparing with those in untreated A549, in JFK treated A549 we observed APA-mediated 3' UTRs alterations in 310 genes including 77 genes with shortened 3' UTRs. In particular, we identified TMEM123, a gene involved in oncotic cell death, which produced transcripts with shortened 3' UTR and thus was upregulated upon JFK treatment. Taken together, our studies suggest that APA might be one of the antitumor mechanisms of JFK and provide a new insight for the understanding of TCM against cancer. PMID: 29234412 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research