Raman micro-spectroscopy analysis of human lens epithelial cells exposed to a low-dose-range of ionizing radiation.

In this study, Raman micro-spectroscopy was used to investigate the effects of varying doses of radiation, ranging from 0.01 Gy to 5 Gy, on human lens epithelial (HLE) cells which were chemically fixed 24 hrs post-irradiation. Raman spectra were acquired from the nucleus and cytoplasm of the HLE cells. Spectra were collected from points in a 3 x 3 grid pattern and then averaged. The raw spectra were preprocessed and principal component analysis followed by linear discriminant analysis (PCA-LDA) was used to discriminate between dose and control for 0.25, 0.5, 2, and 5 Gy. Using leave-one-out cross-validation (LOOCV) accuracies of greater than 74% were attained for each dose/control combination. The ultra-low doses 0.01 and 0.05 Gy were included in an analysis of band intensities for Raman bands found to be significant in the linear discrimination, and an induced repair model survival curve was fit to a band-difference-ratio plot of this data, suggesting HLE cells undergo a nonlinear response to low-doses of IR. A survival curve was also fit to clonogenic assay data done on the irradiated HLE cells, showing a similar nonlinear response. PMID: 29235993 [PubMed - as supplied by publisher]
Source: Physics in Medicine and Biology - Category: Physics Authors: Tags: Phys Med Biol Source Type: research

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Peripheral artery aneurysms (PAAs) include popliteal, femoral, carotid, subclavian, upper limb, and visceral aneurysms, the incidence of which is much lower than that of abdominal aortic aneurysms. The treatment approach most recommended for PAAs is complete resection of the aneurysm sac followed by arterial reconstruction or endovascular repair with a stent graft. Here, we present a new technique, “sleeve shaping,” in the surgical treatment of two complex cases of extracranial carotid and subclavian artery aneurysms.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
The objective of this study was to analyze information from a single clinical center evaluating early results of one-stage endovascular repair for aortic disease and coronary artery bypass graft (CABG) or percutaneous coronary intervention (PCI) for coronary artery disease.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
This study was carried out at Biocor Instituto, Anesthesiology, Nova Lima, MG, Brazil.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
This study developed a fast virtual stenting algorithm to simulate stent-induced aortic remodeling to assist in real-time thoracic endovascular aortic repair (TEVAR) planning and thus to predict complications like distal stent-induced new entry (SINE).
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
Surgical repair of aortic arch aneurysms is still an invasive procedure requiring arch replacement during deep hypothermic circulatory arrest. Endovascular aortic arch repair is less invasive compared with the open surgical aortic arch repair in treating aortic arch disease; this correlates with low morbidity and mortality rates, even in high-risk patients. Aortic arch diseases, such as aneurysms and dissections, often involve the origin of the supra-aortic branches. Surgical aortic arch debranching is therefore indicated before covering of these branches.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
As the preferred method for treatment of type B dissection, thoracic endovascular aortic repair (TEVAR) has been recognized by clinicians for its efficacy compared with open surgery. However, some serious complications still occur after TEVAR, such as progression of anterograde tear of the dissection, formation of new intimal rupture, rupture of the dissection aneurysm, and retrograde type A dissection (RTAD). Although the incidence rate of these complications is not high, the consequence is serious and should cause the clinician's attention.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
Rerouting of the supra-aortic vessels and zone 0 stent grafting have been developed as a less invasive option for aortic arch repair. However, aortic arch aneurysm sometimes involves dilated ascending aorta that cannot provide an appropriate proximal landing zone. We have introduced the ascending aortic banding technique, which reduces aortic diameter to provide sufficient proximal landing zone. The aim of this study was to assess the early and midterm outcomes and anatomic change after the zone 0 stent grafting with ascending aortic banding.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
Open repair of type III thoracoabdominal aneurysms (TAAs) remains a surgical challenge in view of massive dissection and secondary visceral, renal, and limb ischemia during surgery. A novel approach with two-bifurcated graft technique (to reduce visceral, renal, and limb ischemic complications) is reported.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
The bovine aortic arch (BAA) is a common finding in candidates for thoracic endovascular aortic repair, being a marker of thoracic aortic disease. Our aim was to assess whether this peculiar anatomic variant presents a consistent biomechanical pattern of proximal landing zones (PLZs) for thoracic endovascular aortic repair.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
The objective of this study was to evaluate the outcome of the t-Branch (Cook Medical, Bloomington, Ind) stent graft for treatment of thoracoabdominal and pararenal aortic aneurysms in patients who had previous infrarenal aortic repair.
Source: Journal of Vascular Surgery - Category: Surgery Authors: Source Type: research
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