A rapid method to identify Salmonella enterica serovar Gallinarum biovar Pullorum using a specific target gene ipaJ.

In this study, we developed a rapid polymerase chain reaction (PCR) method targeting the specific gene ipaJ to detect S. Pullorum. Among the 650 S. Pullorum strains isolated from 1962 to 2016 all over China, 644 strains were identified to harbor ipaJ gene in the plasmid pSPI12 accounting to a detection rate of 99.08%. Six strains were ipaJ negative because pSPI12 was not found in these strains according to whole genome sequencing results. There was no cross-reaction with other Salmonella serotypes including Salmonella enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum), which show close genetic relationship with S. Pullorum. It means that the PCR method could distinguish S. Gallinarum from S. Pullorum in one-step PCR without complicated biochemical identification. The limit of detection of this PCR method was as low as 90 fg/μL or 102 CFU which showed a high sensitivity. Moreover, this method was applied to identify Salmonella isolated from the chicken farm and the results were consistent with what we obtained from biochemical reactions and serotyping. Together, all the features demonstrated that this one-step PCR method is a simple and feasible method for efficient identification of S. Pullorum. PMID: 29231761 [PubMed - as supplied by publisher]
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research