The ultimate picture-the combination of live cell superresolution microscopy and single molecule tracking yields highest spatio-temporal resolution.

The ultimate picture-the combination of live cell superresolution microscopy and single molecule tracking yields highest spatio-temporal resolution.
Curr Opin Microbiol. 2017 Dec 08;43:55-61
Authors: Dersch S, Graumann PL
Abstract
We are witnessing a breathtaking development in light (fluorescence) microscopy, where structures can be resolved down to the size of a ribosome within cells. This has already yielded surprising insight into the subcellular structure of cells, including the smallest cells, bacteria. Moreover, it has become possible to visualize and track single fluorescent protein fusions in real time, and quantify molecule numbers within individual cells. Combined, super resolution and single molecule tracking are pushing the limits of our understanding of the spatio-temporal organization even of the smallest cells to an unprecedented depth.
PMID: 29227820 [PubMed - as supplied by publisher]

Source: Current Opinion in Microbiology - December 8, 2017 Category: Microbiology Authors: Dersch S, Graumann PL Tags: Curr Opin Microbiol Source Type: research