Sevo flurane induces liver injury by modulating the expression of insulin‐like growth factor 1 via miR‐214

This study aimed to detect the effect of sevoflurane anesthesia on liver injury through modulating IGF‐1. The expression of IGF‐1 and IGF‐1R in liver tissues of sevoflurane‐exposed rats was examined by qRT‐PCR and western blot. The expression levels of miR‐214 in liver cells treated with different concentration of sevoflurane at different time points were detected by qRT‐PCR. Enzyme‐linked immunosorbent (ELISA) assay was used to analyze serum IGF‐1 concentration in cell culture media. After pre‐treatment with 100 nM miR‐214 inhibitor followed by exposure to sevoflurane, the expression level of miR‐214 and IGF‐1 protein in liver cells was examined. Hematoxylin‐Eosin (HE) staining and TUNEL assay was performed to analyze liver tissue necrosis and apoptosis. The expression levels of apoptosis‐related proteins (caspase 3 and Bcl‐xL) were examined using western blot. The mRNA and protein expression level of IGF‐1 and IGF‐1R in rats was significantly down‐regulated after 90 min exposure to sevoflurane. QRT‐PCR results suggested that exposure to sevoflurane upregulated the expression level of miR‐214 and decreased the concentration of IGF‐1 in a dose and time dependent manner. Sevoflurane inhibited the expression of IGF‐1 through up‐regulating miR‐214. IGF‐1 inhibited the positive effect of sevoflurane on cell necrosis and apoptosis. Sevoflurane could induce liver injury by modulating IGF‐1 expression via miR‐214. This art...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research