Effect of polyphenols from coffee and grape on gene expression in myoblasts

Publication date: Available online 23 November 2017 Source:Mechanisms of Ageing and Development Author(s): Alexandros Priftis, Nikolaos Goutzourelas, Maria Chalabalaki, Georgia Ntasi, Dimitrios Stagos, Grigorios D. Amoutzias, Leandros A. Skaltsounis, Dimitrios Kouretas Coffee and grape contain various bioactive compounds like polyphenols that may exert beneficial effects, especially antioxidant activity, on human health upon consumption. However, the molecular mechanisms through which these effects are achieved are not fully elucidated. Thus, in the present study in order to investigate these mechanisms, a whole genome expression DNA microarray analysis was carried out in myoblasts treated with polyphenols of coffee and grape pomace at concentrations that improved the redox status. Grape was composed of catechin, epicatechin, cyanidin, malvidin, delphinidin, petunidin, myrtillin, kuromanin, oenin, peonidin, quercetin, gallic acid and caftaric acid as LC–MS revealed, with a total polyphenolic content (TPC) of 648mg of gallic acid equivalents/g of dry matter. Coffee had a TPC of 42.61mg GAE/g coffee and was composed of 3-chlorogenic acid (16.61mg/g), 4- and 5-chlorogenic acids (13.62mg/g), as UHPLC-HRMS revealed. According to the results, grape polyphenols altered mainly the expression of cytoskeleton and differentiation-associated genes, while coffee compounds had a more profound effect, on the expression levels of many metabolic and antioxidant genes possibly thro...
Source: Mechanisms of Ageing and Development - Category: Geriatrics Source Type: research