Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking.

Total Internal Reflection Fluorescence Microscopy to Study GLUT4 Trafficking. Methods Mol Biol. 2018;1713:151-159 Authors: Wasserstrom S, Morén B, Stenkula KG Abstract Total internal reflection fluorescence (TIRF) microscopy is a powerful method that allows examination of plasma membrane close events in real time. The last decade, the method has successfully been used to explore GLUT4 translocation in adipocytes. Here, we describe the procedure for studying GLUT4 trafficking using TIRF microscopy in isolated primary adipocytes. PMID: 29218524 [PubMed - in process]
Source: Molecular Medicine - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research