Antifungal activity of silver nanoparticles on Fluconazole resistant Dermatophytes identified by (GACA)4 and isolated from primary school children suffering from Tinea Capitis in Ismailia - Egypt.

This study is aimed to detect the antifungal activity of silver nanoparticles (SNPs) on Fluconazole resistant dermatophytes isolated from primary school children clinically suffering from tinea capitis and attending El-Sheikh Zaid Dermatology Center in Ismailia. The study was done on 112 clinical cases. Examination with potassium hydroxide(KOH) of hair samples was done, followed by routine identification using culturing, macroscopical and microscopical examination and biochemical tests, finally molecular identification using Polymerase Chain Reaction (PCR) with (GACA) 4 was done. Fluconazole resistance of these dermatophytes was detected by different methods including agar disc diffusion method and broth microdilution susceptibility testing. Silver nanoparticles susceptibility testing was carried out on these Fluconazole resistant dermatophytes. The Ubiquitin 1 (Ub 1) gene was detected in samples which were Fluconazole resistant but SNPs susceptible. In this study dermatophytes were found only in 70 samples (62.5%). They were belonged to 3 species: Trichophyton violaceum, Microsporum gypseum and Microsporum canis. Fluconazole resistance was found in 58 samples (82.85%). Both M. canis and M. gypseum were resistant to all used concentrations of SNPs, while T. violaceum was susceptible to 50 µg/ml SNPs solution. The Ub1 gene was detected in 1 sample (4.8%). Therefore SNPs can be used for treatment of T. violaceum, while they can't be used for treatment of M. canis or M. gy...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Related Links:

Conclusion: Our estimates indicate that the importance of fungal infections is high but overlooked in Iran, which warrants further actions by health care authorities.
Source: The Journal of Infection in Developing Countries - Category: Infectious Diseases Source Type: research
AbstractPurpose of ReviewThis review summarizes the fungal literature currently available for tinea capitis (TC), as well as providing data for clinical utility.Recent FindingsAvailable studies in TC are scarce; however, they provide important information about efficacy and outcome in clinical practice.SummaryTreatment of TC is effective; however, it requires a minimum of 1  month. Systemic treatment is often required to favor enhance drug penetration into the deep part of the hair follicle. The newest oral antifungal has higher efficacy rates than conventional therapy, as well as much shorter duration of treatment bu...
Source: Current Fungal Infection Reports - Category: Infectious Diseases Source Type: research
Doctors use the skin lesion KOH exam to test a person ’s skin for fungal infections, such as athlete’s foot or ringworm. During the test, they take a skin scraping and place it in a potassium hydroxide (KOH) solution. In this article, we look at the procedure, results, what the KOH exam tests for, and alternatives.
Source: Health News from Medical News Today - Category: Consumer Health News Tags: Dermatology Source Type: news
RINGWORM symptoms include a unique type of rash and other skin changes. The fungal infection is often contracted from walking barefoot on earth, or using swimming pools and communal showers. Watch out for this rash, which is a symptom of the condition.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
The objective of this study was to evaluate the susceptibility of emerging fungi to fluconazole, itraconazole and amphotericin B by disk diffusion method. In 2015, 82 emerging fungi were evaluated in IPB-LACEN/RS and 13 (15.8%) were resistant: 10/52 were from superficial mycoses and 3/30 from systemic mycoses. The data from the study point to the need for permanent vigilance regarding the careful evaluation in the prescription and clinical and laboratory follow-up of patients affected by fungal infections.
Source: Anais Brasileiros de Dermatologia - Category: Dermatology Source Type: research
A look at home remedies for ringworm, a condition that results in fungal infection on the skin. Included is detail on when to see a doctor and prevention.
Source: Health News from Medical News Today - Category: Consumer Health News Tags: Complementary Medicine / Alternative Medicine Source Type: news
AbstractWith the increasing number of immunocompromised hosts, the epidemiological characteristics of fungal infections have undergone enormous changes worldwide, including in China. In this paper, we reviewed the existing data on mycosis across China to summarize available epidemiological profiles. We found that the general incidence of superficial fungal infections in China has been stable, but the incidence of tinea capitis has decreased and the transmission route has changed. By contrast, the overall incidence of invasive fungal infections has continued to rise. The occurrence of candidemia caused byCandida species oth...
Source: Frontiers of Medicine - Category: General Medicine Source Type: research
SKIN and fungal infections - including thrush, ringworm and athlete ’s foot - are caused by different types of fungi.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
Cutaneous infections and infestations are common among children and adolescents. Ectoparasitic infestations affect individuals across the globe. Head lice, body lice, scabies, and infestations with bed bugs are seen in individuals who reside in both resource poor areas and in developed countries. Superficial cutaneous and mucosal candida occur throughput the life cycle. Dermatophyte infections of keratin-containing skin and skin structures result in tinea capitis (scalp), tinea corporis (body), tinea pedis (foot), and tinea unguium (nails).
Source: Current Problems in Pediatric and Adolescent Health Care - Category: Pediatrics Authors: Source Type: research
FUNGAL infections, like athlete ’s foot and ringworm, can usually be easily treated. But invading fungus still kills more people a year than malaria.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
More News: Biology | Children | Dermatology | Egypt Health | Fluconazole | Fungal Infections | Genetics | Molecular Biology | Nanotechnology | Potassium | Ringworm | Skin | Study