Characterization of cultured primary/metastatic lung cancer cells obtained by percutaneous puncture

Conclusions: Primary culture of human lung tumor cells obtained by percutaneous puncture can be successfully achieved with the method described. These primary cultures of cancer cells showed rearrangements of chromosomes and change in their complement chromosomic typical of tumoral cells. Aditionally, Fluorescence in situ Hybridization analysis showed that three cultures showed amplification for EGFR. Finally the expression profiles of the CK7, Napsin A, TTF-1 and P63 genes allowed in most cases to confirm the tumor phenotype of the samples.

Source: European Respiratory Journal - December 6, 2017 Category: Respiratory Medicine Authors: Rojas Moreno, A., Canas Arboleda, A., Herreno, A., Fernandez, M. J., Mejia, J. A., Moreno Nino, O. M., Rey, L., Bustos, F., Montecino, M. Tags: Molecular Pathology and Functional Genomics Source Type: research