Well-defined cationic N-[3-(dimethylamino)propyl]methacrylamide hydrochloride based (co)polymers for siRNA delivery.

Well-defined cationic N-[3-(dimethylamino)propyl]methacrylamide hydrochloride based (co)polymers for siRNA delivery. Biomacromolecules. 2017 Dec 01;: Authors: Singhsa P, Diaz-Dussan D, Manuspiya H, Narain R Abstract Cationic glycopolymers have shown to be excellent candidates for the fabrication of gene delivery devices due to their ability to electrostatically interact with negatively charged nucleic acids and the carbohydrate residues ensure enhanced stability and low toxicity of the polyplexes. The ability to engineer the polymers for optimized compositions, molecular weights and architectures is critical in the design of effective gene delivery vehicles. Therefore, in this study, the aqueous reversible addition-fragmentation chain transfer polymerization (RAFT) was used to synthesize well-defined cationic glycopolymers with various cationic segments. For the preparation of cationic parts, N-[3-(dimethylamino)propyl]methacrylamide hydrochloride (DMAPMA.HCl), water-soluble methacrylamide monomer containing tertiary amine, was polymerized to produce DMAPMA.HCl homopolymer which was then used as macroCTA in the block copolymerization with two other methacrylamide monomers containing different pendant groups, namely 2-aminoethyl methacrylamide hydrochloride (AEMA) (with primary amine) and N-(3-aminopropyl) morpholine methacrylamide (MPMA) (with morpholine ring). In addition, statistical copolymers of DMAPMA.HCl with either AEMA or MPM...
Source: Biomacromolecules - Category: Biochemistry Authors: Tags: Biomacromolecules Source Type: research