Production of an emericellin and its analogues as fungal biological responses for Shimbu-to extract

AbstractThis research examined the production of fungal metabolites as a biological response to Kampo medicines. Shimbu-to (SMB) is a Kampo medicine composed of five herbal components: peony root (Shakuyaku), ginger (Shokyo), processed aconite root (Bushi),Poria sclerotium (Bukuryo), andAtractylodes lancea rhizomes (Sojutsu). High-performance liquid chromatography (HPLC) analysis of the fungusAspergillus nidulans CBS 112.46 incubated in potato dextrose broth supplemented with SMB extract revealed emericellin (2) as the major peak and new xanthone analogues 24-hydroxyshamixanthone (1), shamixanthone (3), epishamixanthone (4), pre-shamixanthone (5), and variecoxanthone A (6) as minor peaks. The structure of1 was determined by detailed analysis of 1D-NMR, 2D-NMR, and MS data. The results suggest that SMB extract regulates the biosynthesis of emericellin and its analogues inA. nidulans. Further investigations revealed that glucose induces the biosynthesis of emericellin and its analogues inA. nidulans in a concentration-dependent manner.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research