Differential Expression of the Aryl Hydrocarbon Receptor and Transforming Growth Factor Beta 1 in Chronic Rhinosinusitis with Nasal Polyps with Allergic Rhinitis

This study aimed to determine the aryl hydrocarbon receptor (AhR) and transforming growth factor beta 1 (TGF- β1) expression levels in chronic rhinosinusitis (CRS) and their possible correlation with allergic state and tissue remodeling.Methods: Patients were enrolled and divided into the following groups: CRS without nasal polyps (NP) without allergic rhinitis (AR) (CRSsNPsAR;n = 20), CRS with NP with AR (CRSwNPwAR;n = 20), CRS with NP without AR (CRSwNPsAR;n = 20), and controls (n = 15). Tissue samples were analyzed by Masson trichrome staining for collagen, while the location and expression of AhR and TGF- β1 were analyzed by immunohistochemistry, quantitative reverse transcription polymerase chain reaction (qRT-PCR), and Western blotting.Results: The collagen amounts as well as AhR and TGF- β1 mRNA and protein expression levels were significantly increased in the CRSsNPsAR group compared with the CRSwNP (CRSwNPsAR and CRSwNPwAR) samples (p
Source: ORL - Category: ENT & OMF Source Type: research