Cellular immune response in MDR-TB patients to different protein expression of MDR and susceptible Mycobacterium tuberculosis : Rv0147, a novel MDR-TB biomarker

AbstractTuberculosis (TB) is a crucial public health problem with prevalence of multidrug resistant (MDR) rising. An accurate TB biomarker is urgently needed to monitor the response to treatment in patients with MDR tuberculosis. To analyze interaction between selected MDR-TB purified protein and immune cells, dendritic cells from MDR-TB patients and healthy subjects were stimulated by 55KDa protein fractions (Rv0147). The purified proteins identified by proteomic techniques (two-dimensional gel electrophoresis, mass spectrometry) and peptide sequences are known to bind a MHC class I alleles which are extracted from the Immune Epitope Database and Analysis Resource database (www.iedb.org). T cells were isolated from PBMC by negative selection and cells were cultured in RPMI-1640 at 37  °C and 5% CO2. Cell culture was assayed for cytokine IL-10 and INF- γ by ELISA. We found that INF-γ production was significantly (335 ± 35.5 pg/ml,P ˂ 0.05) upregulated after protein candidate (Rv0147) stimulation by dendritic cells from MDR-TB patients, whereas IL-10 production was greatly reduced compared with production in healthy subjects (212 ± 9.94 pg/ml,P ˂ 0.05). In fact, the purified protein, Rv0147, stimulated dendritic cells from MDR-TB patients, failed to produce IL-10 and directly stimulates INF-γ production by T cells. These results suggest that the purified protein, Rv0147, may stimulate Th1 type protective cytokine response in MDR-TB p atients but not in...
Source: Immunologic Research - Category: Allergy & Immunology Source Type: research