Diagnostic accuracy of two nucleic acid amplification tests for rapid diagnosis of tuberculous pleural effusion

Source: Respirology - Category: Respiratory Medicine Tags: Tuberculosis 2 Source Type: research

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In this study, we validated the functionality of a powerful Tn mutagenesis tool inMk and used this tool in conjunction with a forward genetic screen to establish a previously unrecognized role of a conserved mycobacterial small RNA gene of unknown function in colony morphology features and biofilm formation. We also combined Tn mutagenesis with next ‐generation sequencing to identify 12,071 Tn insertions that do not compromise viability in vitro. Finally, we demonstrated the susceptibility of theGalleria mellonella larva toMk, setting the stage for further exploration of this simple and economical infection model system ...
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research
AbstractSeveral rapid non-commercial culture-based methods and assays for drug susceptibility testing (DST) ofMycobacterium tuberculosis have emerged over the last decades. The aim of the current review was to summarise evidence on the performance of microscopic observation of drug susceptibility (MODS), thin-layer agar (TLA) and colorimetric redox-indicator (CRI) assays for detection of resistance to first- and second-line anti-tuberculosis (TB) drugs. Forty-three publications satisfying selection criteria were selected for data extraction. MODS and CRI assays demonstrated pooled sensitivity and specificity of> 93% for...
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research
ConclusionVBN is helpful for the diagnosis of TBT-induced CBS and may provide important information on the location, length, diameter and cross-sectional area  of stenosis for further EOB examination and interventional therapy. VBN is recommended for patients with TBT and those with contradictions to bronchoscopy, as well as for regular follow-up of stable TBT, because it reduces the incidence of injury, avoids repeat operations and shortens treatment ti me.
Source: Infectious Diseases and Therapy - Category: Infectious Diseases Source Type: research
ConclusionsChest CT findings would therefore be useful for distinguishing PTB and MACLD when typical findings are observed.
Source: Respiratory Investigation - Category: Respiratory Medicine Source Type: research
(University of Southampton) Researchers at the University of Southampton have identified how new checkpoint inhibitor treatments for cancer can activate tuberculosis in some patients.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
Source: Infection and Drug Resistance - Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research
Cambodia has made notable progress in the fight against tuberculosis (TB). However, these gains are impeded by a significant proportion of undiagnosed cases. To effectively reach people with TB, active case-fi...
Source: Trials - Category: General Medicine Authors: Tags: Study protocol Source Type: research
Previously, we developed a 3-dimensional cell culture model of human tuberculosis (TB) and demonstrated its potential to interrogate the host-pathogen interaction (Tezera et al, 2017). Here, we use the model to investigate mechanisms whereby immune checkpoint therapy for cancer paradoxically activates TB infection. In patients, PD-1 is expressed inMycobacterium tuberculosis (Mtb)-infected lung tissue but absent in areas of immunopathology. In the microsphere model, PD-1 ligands are up-regulated by infection, and the PD-1/PD-L1 axis is further induced by hypoxia. Inhibition of PD-1 signalling increases Mtb growth, and augme...
Source: eLife - Category: Biomedical Science Tags: Immunology and Inflammation Source Type: research
Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an established treatment for hematological malignancies and nonmalignant diseases. Furthermore, haploidentical stem cell transplantation has been developed in the past two decades as another potential strategy for patients with no HLA-matched donor.
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Source Type: research
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