Computational Model of the Effect of a Surface-Binding Site on the Saccharomycopsis fibuligera R64 α-Amylase to the Substrate Adsorption.

The objective of this study was to design a computational model of Sfamy R64 with increased starch adsorptivity using bioinformatics method. The model structure of Sfamy R64 was compared with the positive control, ie, Aspergillus niger α-amylase. The structural comparison showed that Sfamy R64 lacks the surface-binding site (SBS). An SBS was introduced to the structure of Sfamy R64 by S383Y/S386W mutations. The dynamics and binding affinity of the SBS of mutant to the substrate were also improved and comparable with that of the positive control. PMID: 29162975 [PubMed]

https://www.ncbi.nlm.nih.gov/pubmed/29162975?dopt=Abstract

Source: Bioinformatics and Biology Insights - November 23, 2017 Category: Bioinformatics Authors: Yusuf M, Baroroh U, Hasan K, Rachman SD, Ishmayana S, Subroto T Tags: Bioinform Biol Insights Source Type: research