Building better bioorthogonal reactions.

Building better bioorthogonal reactions. Curr Opin Chem Biol. 2014 Jul 30;21C:103-111 Authors: Shih HW, Kamber DN, Prescher JA Abstract Over the past two decades, there has been intense interest in designing and implementing selective (bioorthogonal) reactions for biomolecule tracking. Here we review the most widely used bioorthogonal chemistries in live cells and animals, drawing particular attention to the unique functional groups underlying these transformations. We also describe recent efforts to tune functional group reactivities and stabilities to access even more rapid and selective chemistries. Last, we highlight ongoing challenges in identifying new bioorthogonal reagents and combinations of reactions that can be used concurrently to tag multiple biomolecules. PMID: 25086220 [PubMed - as supplied by publisher]
Source: Current Opinion in Chemical Biology - Category: Biochemistry Authors: Tags: Curr Opin Chem Biol Source Type: research