Fusobacterium nucleatum in gastroenterological cancer: Evaluation of measurement methods using quantitative polymerase chain reaction and a literature review.
Fusobacterium nucleatum in gastroenterological cancer: Evaluation of measurement methods using quantitative polymerase chain reaction and a literature review. Oncol Lett. 2017 Dec;14(6):6373-6378 Authors: Yamamura K, Baba Y, Miyake K, Nakamura K, Shigaki H, Mima K, Kurashige J, Ishimoto T, Iwatsuki M, Sakamoto Y, Yamashita Y, Yoshida N, Watanabe M, Baba H Abstract The human microbiome Fusobacterium nucleatum, which primarily inhabits the oral cavity, causes periodontal disease and has also been implicated in the development of colorectal cancer. However, whether F. nucleatum is present in other gastroenterological cancer tissues remains to be elucidated. The present study evaluated whether quantitative polymerase chain reaction (qPCR) assays were able to detect F. nucleatum DNA and measure the quantity of F. nucleatum DNA in esophageal, gastric, pancreatic and liver cancer tissues. The accuracy of the qPCR assay was determined from a calibration curve using DNA extracted from cells from the oral cavity. Formalin-fixed paraffin-embedded (FFPE) tumor tissues from 20 patients with gastroenterological [esophageal (squamous cell carcinoma), gastric, colorectal, pancreatic and liver] cancer and 20 matched normal tissues were evaluated for F. nucleatum DNA content. The cycle threshold values in the qPCR assay for F. nucleatum and solute carrier organic anion transporter family member 2A1 (reference sample) decreased linearly with the quantity of input DNA (r(2)&...
Date: Thursday, 04 09, 2020; Speaker: Zeynup Gumus, Assistant Professor, Ichan School of Medicine at Mount Sinai; https://cbiit.webex.com/mw3300/mywebex/default.do?
Publication date: Available online 31 March 2020Source: Materials Today: ProceedingsAuthor(s): S. Satheeshchandra, W.N. Namratha, D. Haleshappa, A. Jayarama, Nandakumar Shetty, Richard Pinto
Publication date: Available online 1 April 2020Source: Journal of Membrane ScienceAuthor(s): Lina Xu, Tingting Yang, Mingdi Li, Jiaqi Chang, Jia Xu
Publication date: Available online 31 March 2020Source: CellAuthor(s): Marcus Ruscetti, John P. Morris, Riccardo Mezzadra, James Russell, Josef Leibold, Paul B. Romesser, Janelle Simon, Amanda Kulick, Yu-jui Ho, Myles Fennell, Jinyang Li, Robert J. Norgard, John E. Wilkinson, Direna Alonso-Curbelo, Ramya Sridharan, Daniel A. Heller, Elisa de Stanchina, Ben Z. Stanger, Charles J. Sherr, Scott W. Lowe
Publication date: Available online 31 March 2020Source: Clinica Chimica ActaAuthor(s): Andras Komaromy, Balazs Reider, Gabor Jarvas, Andras Guttman
Publication date: Available online 31 March 2020Source: Clinica Chimica ActaAuthor(s): Yueting Xiong, Chao Shi, Xiaohui Liu, Pengyuan Yang
Authors: Ahmadianpour MV, Mowla J, Sotoodehnejadnematalahi F, Raheb J Abstract Studies on the blood of patients with prostate cancer using Dynamic Light Scattering (DLS) and corona protein size changes have shown that this test is highly specific and sensitive, but this method has not been studied in Iran, and therefore this study intends to perform this procedure using gold nanoparticles in prostate cancer detection. Blood samples of 60 male subjects aged 40-90 years were collected from 20 healthy, 20 benign and 20 prostate cancer patients. Optical scattering changes were measured by the level of gold nanoparticle...
Conclusion: After the third relapse of HGSC, cytotoxic chemotherapy did not prolong survival but was associated with substantially increased healthcare costs. PMID: 32223298 [PubMed - as supplied by publisher]
Publication date: Available online 31 March 2020Source: Journal of Molecular StructureAuthor(s): R. Kaliammal, S. Sudhahar, G. Parvathy, K. Velsankar, K. Sankaranarayanan
Org. Biomol. Chem., 2020, Advance Article DOI: 10.1039/D0OB00059K, PaperAndreas Diacono, Marie Claire Aquilina, Andrej Calleja, Godfrey Agius, Gabriel Gauci, Konrad Szaci łowski, David C. Magri Fluorescent logic gates with benzocrown ethers attached at the imide naphthalimide exhibit synergistic binding of Na+ and K+ in aqueous methanol and water. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
More News: Cancer | Cancer & Oncology | Carcinoma | Colorectal Cancer | Esophagus Cancer | Fusobacterium | Gastric (Stomach) Cancer | Gastroenterology | Hepatocellular Carcinoma | Liver | Liver Cancer | Oral Cancer | Oral Cavity Cancer | Organic | Pancreas | Pancreatic Cancer | Skin Cancer | Squamous Cell Carcinoma | Study | Urology & Nephrology