Indirect tRNA aminoacylation during accurate translation and phenotypic mistranslation.

Indirect tRNA aminoacylation during accurate translation and phenotypic mistranslation. Curr Opin Chem Biol. 2017 Nov 15;41:114-122 Authors: Rathnayake UM, Wood WN, Hendrickson TL Abstract The fact that most bacteria do not contain a full set of aminoacyl-tRNA synthetases (aaRS) is often underappreciated. In the absence of asparaginyl-tRNA and/or glutaminyl-tRNA synthetase (AsnRS and GlnRS), Asn-tRNA(Asn) and/or Gln-tRNA(Gln) are produced by an indirect tRNA aminoacylation pathway that relies on misacylation of these two tRNAs by two different misacylating aaRSs, followed by transamidation by an amidotransferase (GatCAB in bacteria). This review highlights the central importance of indirect tRNA aminoacylation to accurate protein translation, mechanistic peculiarities that appear to be unique to this system, and the newly recognized connection between indirect tRNA aminoacylation and mistranslation as a strategy used by bacteria to respond to environmental stressors like antibiotics. PMID: 29156229 [PubMed - as supplied by publisher]
Source: Current Opinion in Chemical Biology - Category: Biochemistry Authors: Tags: Curr Opin Chem Biol Source Type: research