Facile synthesis of polyaniline-polythionine redox hydrogel: Conductive, antifouling and enzyme-linked material for ultrasensitive label-free amperometric immunosensor toward carcinoma antigen-125.

Facile synthesis of polyaniline-polythionine redox hydrogel: Conductive, antifouling and enzyme-linked material for ultrasensitive label-free amperometric immunosensor toward carcinoma antigen-125. Anal Chim Acta. 2018 Jan 02;997:60-66 Authors: Zhao L, Ma Z Abstract Sensitivity enhancement and proteins adsorption are the common challenges faced in protein immunoassays. In this work, an ultrasensitive and protein-resistant label-free amperometric immunosening platform for carcinoma antigen-125 (CA125) based on redox polyaniline-polythionine hydrogel (PANI-PThi gel) was developed. The as-prepared hydrogel, which was facilely synthesized by electropolymerization, exhibited good conductivity and strong hydrophilicity while the sensitivity and specificity of the immunosensor can be enhanced. Furthermore, the as-prepared AuNPs functionalized PANI-PThi gel exhibited strong current signal and H2O2 electrocatalytic ability, which guaranteed a large current variable range. Based on these, the prepared immunosensor revealed a wide linear range from 0.0001 U mL(-1) to 1 kU mL(-1), a limit of detection of 0.00125 U mL(-1) and its sensitivity was at least three-fold higher than previous works. More importantly, the prepared immunosensor exhibited excellent specificity, making it capable of assaying CA125 in human serum. PMID: 29149995 [PubMed - in process]
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research