Recovery of functionally active trypsin inhibitor (SBTI) and lipoxygenase (LOX) from soymilk via multimodal technique

Publication date: December 2017 Source:Reactive and Functional Polymers, Volume 121 Author(s): Tirunagari Harini, Shalini Basetty, Lakshmi Swarnalatha Jasti, Kuna Lavanya, Nitin W. Fadnavis Commercially available epoxy activated polymers Immobeads and Dilbeads were functionalized to 1,2-amino alcohol derivatives and screened for binding and release of soybean trypsin inhibitor (SBTI) and lipoxygenase (LOX) in soymilk. Moderately hydrophobic polymer DILBEAD-VWR functionalized with diethylamine (DIL-DEA) was found to bind SBTI and LOX (3.5±0.3mg·g−1) without causing protein denaturation. Bound proteins were selectively released by changing the pH of eluting buffer. LOX was obtained in 70±5% overall yield with a purification factor of 5±0.5. The eluent containing SBTI was treated with bovine serum albumin coated polymer (Immobead 350) to obtain SBTI in 61% overall yield and a purification factor of 13.5. The recovered DIL-DEA polymer as well as BSA coated polymer were recycled at least 10 times without any appreciable change in performance.

Source: Reactive and Functional Polymers - November 19, 2017 Category: Chemistry Source Type: research