Roles of N- and C-terminal domains in the ligand-binding properties of cytoglobin.

In this study, we prepared human Cygb mutants (ΔC, ΔN, and ΔNC) with either one or both terminal domains truncated and investigated the enzymatic functions and structural features by spectroscopic methods. Evaluation of the superoxide-scavenging activity between Cygb variants showed that the ΔC and ΔNC mutants exhibited slightly higher activity involving superoxide scavenging as compared with wild-type Cygb. Subsequent experiments involving ligand titration, flash photolysis, and resonance Raman spectroscopic studies suggested that the truncation of the C- and N-terminal domains resulted in less effective to dissociation constants and binding rates for carbon monoxide, respectively. Furthermore, structural stability was assessed by guanidine hydrochloride and revealed that the C-terminal domain might play a vital role in improving structure, whereas the N-terminal domain did not exert a similar effect. These findings indicated that long terminal domains could be important not only in regulating enzymatic activity but also for structural stability, and that the domains might be relevant to other hypothesized physiological functions for Cygb. PMID: 29149638 [PubMed - as supplied by publisher]
Source: Journal of Inorganic Biochemistry - Category: Biochemistry Authors: Tags: J Inorg Biochem Source Type: research

Related Links:

There are limited published data defining survival and treatment response in patients with mild lung disease and/or reduced gas transfer who fulfil diagnostic criteria for idiopathic pulmonary arterial hypertension (IPAH). Patients diagnosed with IPAH between 2001 and 2019 were identified in the ASPIRE (Assessing the Spectrum of Pulmonary Hypertension Identified at a Referral Centre) registry. Using prespecified criteria based on computed tomography (CT) imaging and spirometry, patients with a diagnosis of IPAH and no lung disease were termed IPAHno-LD (n=303), and those with minor/mild emphysema or fibrosis were described...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Pulmonary vascular disease Original Articles: Pulmonary hypertension Source Type: research
Pulmonary hypertension (PH) in the context of chronic lung disease (i.e. group 3 PH) is an ongoing and unresolved challenge. Looking specifically into precapillary PH, it is evident that its presence in chronic lung disease is associated with increased dyspnoea, reduced exercise tolerance, worsened oxygenation, low diffusion capacity of the lung for carbon monoxide (DLCO) and dismal prognosis. Noteably, pulmonary vascular disease is known to negatively impact clinical outcomes in chronic lung disease, even though the definition of precapillary PH may not yet be fulfilled [1]. Vice versa, the presence of mild or subclinical...
Source: European Respiratory Journal - Category: Respiratory Medicine Authors: Tags: Editorials Source Type: research
In a recent issue of Annals, Cho et al investigated the risk of deep venous thrombosis after carbon monoxide poisoning. They found that it was significantly increased by 90 days after carbon monoxide poisoning. The authors used a cohort crossover design in...
Source: SafetyLit - Category: International Medicine & Public Health Tags: Ergonomics, Human Factors, Anthropometrics, Physiology Source Type: news
z D Abstract The use of carbon monoxide as a direct reducing agent for the deoxygenation of terminal and internal epoxides to the respective olefins is presented. This reaction is homogeneously catalyzed by a carbonyl pincer-iridium(I) complex in combination with a Lewis acid co-catalyst to achieve a pre-activation of the epoxide substrate as well as the elimination of CO 2 from a γ-2-iridabutyrolactone intermediate. Especially terminal alkyl epoxides react smoothly and without significant isomerization to the internal olefins under CO atmosphere in benzene or toluene at 80-120 °C. Detailed investigation...
Source: Chemistry - Category: Chemistry Authors: Tags: Chemistry Source Type: research
Conclusions If there is a baby with unexplained neurologic symptoms and signs, CO poisoning should be considered in the differential diagnosis. Early diagnosis and treatment prevent complications and sequelaes.
Source: Pediatric Emergency Care - Category: Emergency Medicine Tags: Illustrative Cases Source Type: research
In this study, various biodiesel blends (Chlorella vulgaris, Jatropha curcus, and Calophyllum inophyllum) were investigated for fuel characteristics, and engine performance with exhaust emission compared to diesel. Chlorella vulgaris, Jatropha curcus, and Calophyllum inophyllum biodiesel were synthesized by the acid–base transesterification approach in a microwave reactor and blended with conventional diesel fuel by volume. The fuel blends were denoted as MB10 (90% diesel + 10% microalgae biodiesel), MB20 (80% diesel + 20% microalgae biodiesel), JB10 (90% diesel + 10% jatropha biodiesel), JB20 (80% diesel + 20% j...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
Publication date: Available online 30 May 2020Source: The Journal of Chemical ThermodynamicsAuthor(s): Antonin Chapoy, Pezhman Ahmadi, Valdério de Oliveira Cavalcanti Filho, Prashant Jadhawar
Source: The Journal of Chemical Thermodynamics - Category: Chemistry Source Type: research
Physiological Reports, Volume 8, Issue 11, June 2020.
Source: Physiological Reports - Category: Physiology Authors: Tags: LETTER TO THE EDITOR Source Type: research
Publication date: June 2020Source: Annals of Emergency Medicine, Volume 75, Issue 6Author(s): Yifang Xu, Wenchen Li
Source: Annals of Emergency Medicine - Category: Emergency Medicine Source Type: research
New J. Chem., 2020, Advance Article DOI: 10.1039/D0NJ01500H, PaperMingming Luo, Zhao Liang, Mingwei Chen, Shaik Gouse Peera, Chao Liu, Hui Yang, Xiaopeng Qi, Juan Liu, Tongxiang Liang CO oxidation on MnC3 and MnC4 has fast kinetics and a low energy barrier. To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - New J. Chem. latest articles - Category: Chemistry Authors: Source Type: research
More News: Biochemistry | Carbon Monoxide Poisoning | Study