Extraction, purification of Prostate Specific Antigen (PSA) and establishment of radioimmunoassay system as a diagnostic tool for prostate disorders.

This study aimed to provide an easy and effective method for extraction and purification of prostate specific antigen (PSA) from human seminal fluid with high quantity (14 mg) and high purity (98%). The obtained PSA was injected into rabbits for production of anti-PSA polyclonal antibody (titer 1/1000), labeled with radioactive iodine-125 for preparation of radioactive PSA tracer (purity 98 ± 1.8% and specific activity 64 ± 1.9 µCi/µg), and used in preparation of PSA standards. All prepared components can be used in PSA immunoassays specially radioimmunoassay (RIA) kit preparation as a diagnostic tool for prostatic diseases.
PMID: 29144195 [PubMed - as supplied by publisher]

Source: Journal of Immunoassay and Immunochemistry - November 17, 2017 Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research