Evaluation of UMELISA( ®) T4 Neonatal and UMELISA(®) T4 using polystyrene plates coated with anti-thyroxine (T4) monoclonal antibodies.

Evaluation of UMELISA(®) T4 Neonatal and UMELISA(®) T4 using polystyrene plates coated with anti-thyroxine (T4) monoclonal antibodies. J Immunoassay Immunochem. 2017 Nov 16;: Authors: Castells EM, González EC, Pérez PL, Del Río L, Tejeda Y, Perea Y, Martín O, Espinosa M, Rivero JA, Frómeta A Abstract Congenital hypothyroidism is one of the most common preventable causes of mental retardation. The Center of Immunoassay has developed the UMELISA(®) T4 NEONATAL and UMELISA(®) T4 to determine neonatal T4 levels in dried blood and serum samples. Both reagent kits use the same polystyrene plates coated with anti- thyroxine (T4) polyclonal antibodies as solid phase. This work shows the re-standardization of the UMELISA(®) T4 NEONATAL and UMELISA(®) T4 using plates coated with anti-T4 monoclonal antibodies (T4Mabs). Polystyrene plates of the modified assays were firstly coated with polyclonal IgG sheep-anti-mouse IgG for 18 hours. T4Mabs were added to the plates and incubated for 2 hours at room temperature. Different performance parameters were evaluated and correlation studies with the commercial kits done. Using polystyrene plates coated with T4Mabs increases the slope of the calibration curve in the clinical interest zone. The assay conjugates work twice diluted in respect to the ones of the commercial kits. Recovery percentages (90.8-110.7 for UMELISA(®) T4 NEONATAL and 92.1-109.3 for UMELISA(®) T4) and intra (7.2-7.6 for...
Source: Journal of Immunoassay and Immunochemistry - Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research