Disruption of AT-hook 1 domain in MeCP2 protein caused behavioral abnormality in mice

In this study, we generated a mouse line carrying deletion of eight conserved amino acids in AT-hook 1 domain by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technology. Mecp2 ΔAT-hook1/y mutant male mice exhibited low locomotor activity, motor incoordination and cognitive deficit. In addition, these mutant mice exhibited increased anxiety. Moreover, pain insensitivity was noted in the mutant males. However, the social interactions were unaffected in AT-hook 1 mutant mice. Thinner CA1 region of the hippocampus was observed in the mutant mice. On the molecular basis, Western blot analysis showed increased expression of mutant MeCP2 protein in the cortex. Additionally, several genes expressed specifically in inhibitory neurons were markedly changed in the cerebrum. Taken together, these data demonstrate that disruption of AT-hook 1 domain in MeCP2 caused behavioral abnormality in mice, which suggests that AT-hook 1 is a critical region for the function of MeCP2 protein.
Source: Biochimica et Biophysica Acta (BBA) Molecular Basis of Disease - Category: Molecular Biology Source Type: research

Related Links:

Publication date: January 2020Source: Food Research International, Volume 127Author(s): Sandra Machado Lira, Ana Paula Dionísio, Marcelo Oliveira Holanda, Chayane Gomes Marques, Gisele Silvestre da Silva, Lia Coêlho Correa, Glauber Batista Moreira Santos, Fernando Antonio Pinto de Abreu, Francisco Ernani Alves Magalhães, Emanuela de Lima Rebouças, Jhonyson Arruda Carvalho Guedes, Danielle Ferreira de Oliveira, Maria Izabel Florindo Guedes, Guilherme Julião ZocoloAbstractPitaya is a Cactacea with potential for economic exploitation, due to its high commercial value and its functional compon...
Source: Food Research International - Category: Food Science Source Type: research
CONCLUSION: There is currently no uniform hospitalization strategy for postoperative care of children who undergo typical otorhinolaryngologic interventions in Germany. PMID: 31712876 [PubMed - as supplied by publisher]
Source: HNO - Category: ENT & OMF Tags: HNO Source Type: research
CONCLUSION: CRP SNPs rs1800947 and rs2794521 may be associated with depressive symptoms in patients with depression in a sexspecific fashion. Furthermore, rs2794521 may be a predictor of the efficacy of antidepressants in female patients. PMID: 31711279 [PubMed - as supplied by publisher]
Source: Psychiatry Investigation - Category: Psychiatry Tags: Psychiatry Investig Source Type: research
High blood pressure? There ’s an app for that. Diabetes? There’s an app for that, too. Want to quit smoking? Keep track of your exercise? Is your anxiety getting the better of you? There are apps for all of those health issues, hundreds of thousands of apps. Developers at Blue Shield of California have rolled out a ne w, enhanced Wellvolution website — Wellvolution Next — that brings together roughly 40 of the best “digital therapeutics” applications in one place where members can…
Source: bizjournals.com Health Care:Biotechnology headlines - Category: Biotechnology Authors: Source Type: news
ConclusionThe acute toxicity associated with oral selenium ingestion and the blood and urinary levels of selenium in different cases of poisonings were reviewed. Mortality is a risk of acute selenium poisoning. Concentrations of selenium in blood and urine samples in non-fatal cases are close to those observed in fatal cases.
Source: Journal of Trace Elements in Medicine and Biology - Category: Biochemistry Source Type: research
Even back in 2005 when Making Peace With Autism came out, I knew that I’d made a terrible mistake with the opening sentence: “The hardest day of the year for me is Nat’s birthday, November 15.” How could I have thought such a thing, much less published it for everyone to see? What if Nat saw? Realistically, he wouldn’t because he doesn’t read books like that. But still. Imagine a child seeing that. Further on I wrote: Of course, Ned and I are happy to celebrate Nat’s birthday, going to great lengths to come up with presents that catch his quicksilver attention and baking a cake sla...
Source: Susan's Blog - Category: Child Development Authors: Tags: Uncategorized Source Type: blogs
The meet-cute is neatly postponed in this entertaining story of two next-door neighbours, both seeing therapists and looking for loveMuch like the process of psychotherapy itself, patience is required for results while watching this modestly entertaining slow-burn Parisian romance fromC édric Klapisch, who pulls off a neat trick by bumping the meet-cute to the end of the film.What happens before his protagonists clap eyes on each other is a kind of emotional spring clean. Both of them, a man and woman in their early 30s, are struggling with anxiety and stress; they find their way to therapy and sift through their se...
Source: Guardian Unlimited Science - Category: Science Authors: Tags: Romance films Psychology France World news Science Europe Culture Paris Source Type: news
Authors: Morris AL, Colbourne T, Kirkpatrick I, Banerji V Abstract Nontraumatic chylous pleural effusions (chylothorax) and pericardial effusions (chylopericardium) are rare. They can, however, accompany intrathoracic malignancies and, most commonly, lymphomas. An association of chronic lymphocytic leukemia (cll) with chylopericardium has rarely been reported. A 68-year-old woman with cll, previously treated with single-agent fludarabine in the community, developed pleuritic chest pain and a new pericardial effusion. Computed tomography (ct) imaging of her chest revealed a large pericardial effusion with progressiv...
Source: Current Oncology - Category: Cancer & Oncology Tags: Curr Oncol Source Type: research
ConclusionsPenetrating trauma victims are at increased risk of returning to the ED, most often because of wound or pain issues. Recognizing these factors allows for targeted interventions to decrease ED resource utilization.
Source: The American Journal of Surgery - Category: Surgery Source Type: research
ConclusionMesenteric cysts represent a rare cause of intra-abdominal mass. Owing to low prevalence, literature is limited as is guidance on management. Careful pre-operative planning is essential so as to avoid operative complications. Imaging such as USS is of great importance; however Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) may be of more benefit, owing to the complex anatomical relations within which mesenteric cysts can present. Surgical excision is widely reported as the surgical treatment of choice for symptomatic cysts
Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
More News: Anxiety | Autism | Brain | Databases & Libraries | Genetics | Girls | Molecular Biology | Neurology | Pain | Rare Diseases | Rett Syndrome | Study