Single mRNA Molecule Detection in Drosophila.

Single mRNA Molecule Detection in Drosophila.
Methods Mol Biol. 2018;1649:127-142
Authors: Little SC, Gregor T
Abstract
Single molecule fluorescent in situ hybridization (smFISH) enables quantitative measurements of gene expression and mRNA localization. The technique is increasingly popular for analysis of cultured cells but is not widely applied to intact organisms. Here, we describe a method for labeling and detection of single mRNA molecules in whole embryos of the fruit fly Drosophila melanogaster. This method permits measurements of gene expression in absolute units, enabling new studies of transcriptional mechanisms underlying precision and reproducibility in cell specification.
PMID: 29130194 [PubMed - in process]

Source: Mol Biol Cell - November 14, 2017 Category: Molecular Biology Authors: Little SC, Gregor T Tags: Methods Mol Biol Source Type: research