In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes.

In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes.
Methods Mol Biol. 2018;1649:273-287
Authors: Chamiolo J, Gaspar I, Ephrussi A, Seitz O
Abstract
Fluorogenic hybridization methods, such as the use of FIT probes, enable the in vivo detection of specific mRNAs transcribed from their endogenous, genetically nonmodified loci. Here, we describe the design, synthesis and injection of nuclease resistant FIT probes into developing Drosophila oocytes to detect endogenous localizing mRNAs as wells as to probe function of structural RNA elements.
PMID: 29130204 [PubMed - in process]

Source: Mol Biol Cell - November 14, 2017 Category: Molecular Biology Authors: Chamiolo J, Gaspar I, Ephrussi A, Seitz O Tags: Methods Mol Biol Source Type: research