Comparative transcriptomic characterization of a new mib mutant allele, mib(nn2002), in zebrafish.

Comparative transcriptomic characterization of a new mib mutant allele, mib(nn2002), in zebrafish. Gene. 2017 Nov 07;: Authors: Lai KP, Li JW, Hsu CH, You MS, Chan TF, Tse WKF, Jiang YJ Abstract mib(nn2002), identified from an allele screen, shows early segmentation defect and severe cell death phenotypes, which are different from those of other described mib mutant alleles. We have previously reported its defects in somitogenesis and identified its origin of mutation, a large deletion in LG2. The report here is a continuous study, where we applied the bioinformatics analysis to profile the genetic background of mib(nn2002) mutants. By comparing the transcriptomic data of mib(nn2002) mutants with those of AB wild-type, a total of 1945 differentially expressed genes were identified, including 685 up- and 1260 down-regulated genes. The Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis and Ingenuity Pathway Analysis (IPA) identified the enriched pathways and their related biological functions. Our data further demonstrated that the defects in the somitogenesis were related to the down-regulated segmentation genes, such as foxc1a, smyhc1, myod1 and mylpfa. PMID: 29126925 [PubMed - as supplied by publisher]
Source: Gene - Category: Genetics & Stem Cells Authors: Tags: Gene Source Type: research