Beyond D-luciferin: expanding the scope of bioluminescence imaging in vivo.

Beyond D-luciferin: expanding the scope of bioluminescence imaging in vivo.
Curr Opin Chem Biol. 2014 Jul 28;21C:112-120
Authors: Adams ST, Miller SC
Abstract
The light-emitting chemical reaction catalyzed by the enzyme firefly luciferase is widely used for noninvasive imaging in live mice. However, photon emission from the luciferase is crucially dependent on the chemical properties of its substrate, D-luciferin. In this review, we describe recent work to replace the natural luciferase substrate with synthetic analogs that extend the scope of bioluminescence imaging.
PMID: 25078002 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - July 28, 2014 Category: Biochemistry Authors: Adams ST, Miller SC Tags: Curr Opin Chem Biol Source Type: research