Antibody Selection on FFPE Tissue Slides.

Antibody Selection on FFPE Tissue Slides.
Methods Mol Biol. 2018;1701:381-391
Authors: Ten Haaf A, Gattenlöhner S, Tur MK
Abstract
Standard antibody phage-display panning uses purified proteins, antigen-transfected cells, or tumor cell lines as target structure to generate specific antibodies. Here, we describe a method for the selection of specific antibodies by phage panning against routine formalin-fixed paraffin-embedded (FFPE) tissue biopsies immobilized on glass slides. Selected antibody fragments recognize disease-associated antigens in its native conformation, suitable for the development of targeted diagnostic and therapeutic agents.
PMID: 29116517 [PubMed - in process]

Source: Mol Biol Cell - November 10, 2017 Category: Molecular Biology Authors: Ten Haaf A, Gattenlöhner S, Tur MK Tags: Methods Mol Biol Source Type: research