Rapid screening, separation, and detection of α-glucosidase inhibitors from Hedyotis diffusa by ultrafiltration–liquid chromatography tandem mass spectrometry–high-speed countercurrent chromatography

In this study, a new assay based on ultrafiltration –liquid chromatography was developed for the rapid screening and identifying of ligands for α-glucosidase inhibitors fromHedyotis diffusa. Eleven potential α-glucosidase inhibitors were found in the crude extract, and four of these were separated successfully by high-speed countercurrent chromatography: 2-hydroxyl-3-methyl anthraquinone, 2-methyl-3-hydroxyl anthraquinone, 2-hydroxyl-3-methyl-1-methoxyl anthraquinone, and a novel compound. The percent purities of the compounds are 97.71, 95.96, 87.60, and 93.66%, respectively. Results of our study demonstrated that our systematic screening, identification, and isolation of bioactive components inHedyotis diffusa using ultrafiltration –liquid chromatography–diode array detector, liquid chromatography–electrospray ionization–tandem mass spectrometry, and high-speed countercurrent chromatography are feasible and efficient. Our approach also can be used to rapidly screen and separate other enzyme inhibitors.
Source: Medicinal Chemistry Research - Category: Chemistry Source Type: research